Published on in Vol 10, No 4 (2021): April

Preprints (earlier versions) of this paper are available at, first published .


1Universidade Federal de Minas Gerais, Belo Horizonte, Brazil

2Rheumatology Department, Hospital Universitário Evangélico Mackenzie, Paraná, Brazil

3Rheumatology Department, Santa Casa de Misericórdia de São Paulo, São Paulo, Brazil

4Rheumatology Department, Universidade Federal do Amazonas, Manaus, Brazil

5Medical School, Universidade de São Paulo, São Paulo, Brazil

6Medical School, Centro Universitário de Brasília, Brasília, Brazil

7Rheumatology Department, Universidade Federal de Pernambuco, Recife, Brazil

8Rheumatology Department, Universidade Federal de Fortaleza, Fortaleza, Brazil

9Rheumatology Department, Hospital do Servidor Público Estadual, Instituto de Assistência Médica ao Servidor Público Estadual, São Paulo, Brazil

10Rheumatology Department, Universidade de Brasília, Brasília, Brazil

11Rheumatology Department, Universidade Federal de São Paulo, São Paulo, Brazil

12Rheumatology Department, Universidade Federal do Paraná, Curitiba, Brazil

13Rheumatology Department, Faculdade de Ciências da Saúde de Barretos, Barretos, Brazil

14Rheumatology Department, Pontifícia Universidade Católica de Campinas, Campinas, Brazil

15Rheumatology Department, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil

16see Acknowledgments

*all authors contributed equally

Corresponding Author:

Débora Cerqueira Calderaro, MD, PhD

Universidade Federal de Minas Gerais

190 Professor Alfredo Balena Avenue

Belo Horizonte, 30130-100


Phone: 55 3134099757


Background: The COVID-19 pandemic has resulted in social isolation, which has a potential negative impact on the educational routines (eg, the suspension of face-to-face appointments) and mental health of medical students. The Mario Pinotti II (MPII) study is a 24-week observational study that conducted scheduled telephone calls every 2 weeks to verify the occurrence of COVID-19 in patients with rheumatic diseases on chronic hydroxychloroquine therapy (from March 29, 2020, to September 30, 2020). The effects of voluntarily participating in a research project (ie, one that involves interactions via telephone contact with patients, professors, rheumatologists, and colleagues) on the daily lives and mental health of medical students requires evaluation.

Objective: As medical students are professionals in training and have a high level of responsibility in terms of handling the emotional and physical aspects of several diseases, this study aims to evaluate the impacts of the COVID-19 pandemic and participation in the MPII study on the educational routines and mental health of medical students.

Methods: A web-based survey was carried out to perform a cross-sectional comparative assessment of medical students who participated in the MPII study and their colleagues who were not involved in the MPII study. Participants from both groups were matched based on sex, age, and medical school. The web questionnaire was developed by a panel composed of graduate medical students, rheumatologists, medical school professors, and a psychology professor. The questionnaire included details on demographic and life habits data and evaluated participants' impressions of the MPII study and the impact of the COVID-19 pandemic on their educational routines and medical training. In addition, depression, anxiety, and stress were evaluated using the Brazilian version of the Depression, Anxiety, and Stress Scale (DASS)-21, and currently, the DASS-21 scores are grouped as those that indicate a low, moderate, or high risk of mental distress. This project was approved by the Federal University of São Paulo Ethics Committee (CAAE: 34034620.0.0000.5505).

Results: Data were collected from both medical student groups from July 20 to August 31, 2020. Data extraction was completed in September 2020. The data analysis is ongoing. We expect the results to be published in the first semester of 2021.

Conclusions: This study will provide insight into the effects of participating in a research project on depression, anxiety, and stress, which will be determined by applying the DASS-21 to a large sample of Brazilian undergraduate medical students. We will also evaluate the impact of the COVID-19 pandemic on medical students’ educational routines and medical training.

International Registered Report Identifier (IRRID): DERR1-10.2196/24617

JMIR Res Protoc 2021;10(4):e24617



In December 2019, a respiratory disease (COVID-19) that is caused by the novel SARS-CoV-2 was identified in Wuhan City, Hubei Province, China. Several weeks later, the World Health Organization declared COVID-19 as an international public health emergency and pandemic [1].

Given the high levels of community transmission of SARS-CoV-2, several approaches have been recommended to mitigate global viral spread; social distancing, quarantine, intermittent hand hygiene, and universal masking are especially important approaches. However, these government recommendations vary according to each country and have resulted in home isolation, fear, uncertainty, anxiety, depression, high alcohol intake, domestic violence, education impairment, and severe economic burdens [2-4].

With regard to medical education, the majority of medical classes and face-to-face practices were suspended for a long period while medical schools prepared for remote education. Furthermore, several students have been invited to work on the front line of the pandemic (ie, treating patients with COVID-19)), which allowed them to graduate earlier [5,6]. These measures, which are associated with remote (not in-person) training, have hampered medical training for working with patients and other relevant aspects, including hospital and outpatient clinics and regulatory processes [7]. Several researchers have highlighted a gradual increase in anxiety levels among medical students during the COVID-19 pandemic, suggesting that the pandemic may be impairing several aspects of social relationships, technical performance, and mental health [7,8].

It is worth emphasizing that medical training is often exhausting due to specific technical requirements and a large number of stressful factors [9], including full-time dedication, personal life–related effort and sacrifice, close contact with severe diseases and death, and physical and emotional distress [10]. During the COVID-19 pandemic, extensive and intense workloads, difficulties in reconciling personal life with studies, competitiveness, sleep deprivation, fears of making mistakes and getting sick, tiredness, and decision making under pressure may result in high levels of anxiety and depression in medical students [9-13].

European studies have reported that around 30% of medical students experience some level of depression or anxiety. In Brazil, studies have suggested that 20%-50% of medical students experience mood changes [12]. In addition, depression and suicidal ideation rates are higher in medical students than in the general population, and these students generally seek less help from psychological or psychiatric professionals [5-12]. Several psychiatric illnesses and personality disturbances have been reported to be related to such behavior, including eating disorders, the denial of reality, alcoholism, the abuse of illicit drugs, a lack of commitment, obsessive-compulsive disorder, anxiety, depression, and increased suicide rates [7]. Thus, medical students are susceptible to experiencing inadequate or nonadaptive responses to emotional distress [7,13].

Since January 2020, the World Health Organization has been warning the public that the COVID-19 pandemic is generating stress in the general population [1], especially stress related to uncertainties about the course and prognosis of the disease; fear; a lack of resources for diagnosis and treatment; a shortage of food, medication, and adequate supplies of personal protective equipment for health care professionals; feelings of missing the freedom of travel; and conflicting information delivered by governmental authorities or social media [14-16]. It has also been reported that the incidence of several psychological disorders increased during previous pandemics, including the SARS (Severe Acute Respiratory Syndrome and MERS (Middle Eastern Respiratory Syndrome) pandemics. Such disorders mainly included anxiety and depression [8].

During the COVID-19 pandemic in China, a study that evaluated 217 medical students reported that depression and anxiety occurred in 35% and 22% of students, respectively [8]. Furthermore, researchers at medical schools in Wisconsin believe that students are great allies of doctors [7].

The Mario Pinotti II (MPII) study is a noninterventional, observational, multicenter, parallel-group cohort study that included adult volunteers (aged ≥18 years) with a previously known diagnosis of rheumatic disease. These participants were on hydroxychloroquine for at least 30 days prior to baseline. The MPII is a 24-week prospective study that included more than 10,000 individuals from 20 centers in Brazil. A total of 6 sequential telephone calls were scheduled during the community transmission of SARS-CoV-2. These calls were performed by 395 volunteer medical students [17].

Given the close social interaction between the patients and controls, which was facilitated through periodic telephone contact, as well as the social interactions among principal investigators, study coordinators, and professors, our main hypothesis was that medical students who participated in the MPII study would experience less emotional distress than their colleagues who did not participate in the MPII study. [12-15].

Our objectives were to evaluate the impact of participating in the MPII study on the mental health (evaluated using the Depression, Anxiety, and Stress Scale [DASS]-21) [18,19], professional improvement, and commitment perceptions of medical students during the COVID-19 pandemic. We also aimed to identify potential impairments in the educational routines of students’ medical schools and to report on COVID-19 diagnoses among this population.

Study Design

We will conduct a comparative, cross-sectional, observational, case-control study that used a voluntary web-based survey. The survey was conducted according to the Checklist for Reporting Results of Internet E-Surveys (CHERRIES) statement [20].

Study Population

Medical students who were involved in the MPII study (volunteer group) and their colleagues who were not involved in the MPII study (control group) [17] were recruited.

Sample Size

Convenience sampling was conducted based on voluntary involvement, as per the investigators in the MPII Study. Of the 20 MPII centers, 14 (70%) participated in this study. All students who successfully answered the web questionnaire during the data collection period were included in this study.

Inclusion Criteria

The volunteer group consisted of medical students who participated as volunteers in the MPII study and were aged ≥18 years. The control group consisted of medical students who did not participate in the MPII study. For each participant in the volunteer group, at least two other students were enrolled as controls. This was done to ensure that the number of participants was sufficient for stratified analysis, as we expected that the prevalence of mental health issues among medical students would be high [21].

An electronic informed consent form was provided to both groups, and participants were required to sign it before they were granted access to the survey questionnaire.

Exclusion Criteria

Participants were excluded if they refused to provide informed consent or withdrew their consent.

Survey Questionnaire

This study was approved by the Federal University of São Paulo Ethics Committee (Certificado de Apresentação de Apreciação ética [CAAE]: 34034620.0.0000.5505) on July 13, 2020.

The complete survey questionnaire form can be found in Multimedia Appendix 1. It includes 69 questions. The time required for completing the survey was 20 minutes. The questionnaire was developed and provided to participants in Portuguese (their mother language), and it was only translated in order to be published. The translation was verified by an experienced translator in Brazil.

A panel of undergraduate medical students, rheumatologists, and medical school professors who were involved in the MPII study, as well as a psychology professor who had experience in conducting web-based surveys to evaluate the mental health of medical students and health care professionals, were responsible for developing the web-based survey questionnaire. The students in the panel that developed the survey (n=3) tested the questionnaire. Afterward, it was distributed to the other participants of this study.

The volunteer group received an invitation video that provided explanations about the survey and the link to access the web questionnaire. Subsequently, the volunteers were requested to send an invitation link to colleagues who were not participating in the MPII study. These colleagues were added to the control group. No identification data were requested.

An informed consent form was integrated into the web questionnaire. Participants were required to provide their consent to participate in the survey (electronic informed consent) before accessing the questionnaire of the study or providing any information.

Participants can provide an email address if they want to receive their mental health evaluation results. Participants or researchers who require a psychological or psychiatric evaluation will be provided (through email contact) with guidance on accessing local facilities that offer these services. All participants were informed that providing an email address is voluntary and can be done before answering the survey questionnaire.

Demographic and epidemiologic data and details about comorbidities, life habits (smoking, alcohol intake, illicit drug use, and physical activity), and concomitant medications were recorded. In addition, specific aspects related to medical schools, such as the type of school (public or private), costs, teaching activities, and feelings about medical training during the pandemic, were addressed. With regard to participants in the volunteer group, their impressions of the procedures in the MPII study and the study’s impact on their daily routines were evaluated.

The Brazilian version of the DASS-21 was used to evaluate mental health [18,19]. The DASS-21 is a set of three self-report scales with seven items each. The items were designed to measure emotional status. The depression domain assesses dysphoria, hopelessness, the devaluation of life, self-deprecation, a lack of interest or commitment, anhedonia, and inertia. The anxiety domain evaluates autonomic symptoms, skeletal muscle effects, coping, and experiences. The stress domain is sensitive to levels of chronic nonspecific arousal, and it assesses difficulties in relaxing; nervous arousal; and the state of being easily upset/agitated, irritable/overreactive, and impatient. Scores for depression, anxiety, and stress are calculated by summing the scores of the relevant items. The DASS-21 is based on a dimensional concept of psychological disorders instead of a categorical concept of psychological disorders, and it was developed by accounting for the differences among the depression, anxiety, and stress experienced by subjects. Therefore, the scale does not have any direct implications for diagnosis.

DASS-21 scores were grouped as those that indicate a low, moderate, or high risk or mental distress. These will be stratified according to gender and the SD from the study population’s mean scores (low: lower than mean+1 SD; moderate: ranges from mean+1 SD to mean+2 SDs; high: greater than mean+2 SDs).

Recommended cutoff scores for conventional severity labels of depression, anxiety, and stress (normal, mild, moderate, severe, and extremely severe) were multiplied by 2, as shown in Table 1 [19].

Table 1. Cutoff scores for depression, anxiety, and stress according to the Depression, Anxiety, and Stress Scale (DASS)-21.
Severity labelDoubled DASS-21 domain scores [19]

Extremely severe≥28≥20≥34

Data Collection

Data collection was performed by using a web questionnaire that was generated on the Google Forms platform. The questionnaire was disclosed to the research subjects via email and WhatsApp (WhatsApp LLC). The data collection period was from July 20 to August 31, 2020.

Statistical Analysis

Descriptive analysis will be performed using absolute and relative frequencies for categorical variables and quantitative measures (means, quartiles, minimums, maximums, and SDs) for numerical variables. The normality of numerical variables will be evaluated using the Kolmogorov-Smirnov test. Numerical variables with normal distributions will be described as mean (SD), and nonnormal numerical variables will be described as median (IQR) or range (minimum-maximum).

The Chi-square association test with adjusted standardized residuals will be used to assess the association between categorical variables; the Fischer exact test will be used for small samples. The linear associations between two numerical variables will be evaluated using the Pearson or Spearman correlation method.

The comparison between the mean numerical variables with normal distributions in the volunteer group and those in the control group will be conducted by using the Student t test. If the assumption of normality is violated, the Mann-Whitney nonparametric test will be used.

Adjusted multiple linear regression models will be used to assess the simultaneous effects of sex, age, comorbidities, concomitant medications, and other confounding variables based on group type and predefined outcomes (anxiety, depression, and stress scores from the DASS-21). For dichotomous dependent variables, a logistic regression model will be used.

SPSS, version 20 (IBM Corporation) will be used for all analyses. A P value of <.05 will be considered significant.

Data were collected from both medical student groups from July 20 to August 31, 2020. Data extraction was completed on September 2020. The data analysis is ongoing. We expect the results to be published in the first semester of 2021.

This study has an unprecedented design, as it includes a very large sample of volunteer medical students from 14 Brazilian tertiary rheumatology centers. These students are currently monitoring the outcomes of 9589 patients with rheumatic diseases on hydroxychloroquine and assessing patients’ susceptibility to SARS-CoV-2 infection.

The main objective of this study is to evaluate the impact of participating in a research project during the COVID-19 outbreak on the mental health and learning behaviors of medical students. These variables were measured by using a structured web questionnaire about students’ volunteer participation in the MPII study and their ability to work with patients and professors in a real-life scenario.

This study has several innovative aspects, such as (1) the evaluation of depression, anxiety, and stress by applying the DASS-21 to a large sample of medical students and a control group; (2) medical students' impressions regarding the handling of the uncertainty and doubts of patients with rheumatic diseases, including the fear of illness, fear of dying, and shortage of medication during the outbreak; (3) the measurement of the impact of the COVID-19 pandemic on students’ educational routines and medical training; and (4) the fact that the web questionnaire was developed by a panel composed of graduate medical students, rheumatologists, medical school professors, and a psychology professor.


We would like to acknowledge Faculdade de Medicina de Barretos, Education & Research Institute Cancer Hospital Barretos, Barretos-SP, Brazil; Hospital das Clínicas, Universidade Federal de Minas Gerais, Belo Horizonte-MG, Brazil; Hospital Universitário de Brasília da Universidade de Brasília, Empresa Brasileira de Serviços Hospitalares, Brasília-DF, Brazil; Hospital São Paulo da Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo-SP, Brazil; Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro-RJ, Brazil; Hospital do Servidor Público Estadual, Instituto de Assistência Médica ao Servidor Público Estadual, São Paulo-SP, Brazil; Hospital Universitário Lauro Wanderley, Universidade Federal da Paraíba, João Pessoa-PB, Brazil; Hospital das Clínicas da Universidade Federal de Pernambuco, Recife-PE, Brazil; Hospital Universitário da Universidade Federal de Juiz de Fora, Juiz de Fora-MG, Brazil; Hospital Geral de Fortaleza, Fortaleza-CE, Brazil; Hospital Universitário Pedro Ernesto, Universidade do Estado do Rio de Janeiro, Rio de Janeiro-RJ, Brazil; Irmandade da Santa Casa de Misericórdia de São Paulo, São Paulo-SP, Brazil; Universidade Federal do Paraná, Curitiba-PR, Brazil; Hospital Universitário Getúlio Vargas Universidade Federal do Amazonas, Manaus-AM, Brazil; Hospital Universitário Evangélico Mackenzie, Curitiba-PR, Brazil; and Hospital Universitário Cassiano Antonio de Moraes, Universidade Federal do Espírito Santo, Vitória-ES, Brazil. This paper was written on behalf of The Voluntary Brazilian Society of Rheumatology Task Force Against COVID-19.

The members of The Voluntary Brazilian Society of Rheumatology Task Force Against COVID-19, Mirhelen Abreu, Cleandro Albuquerque, Ana Bacchiega, Blanca Bica, Danielle Brito, Ângela Duarte, Paula Fernandes, Ana Guedes, Evandro Klumb, Helena Pereira, Emília Sato, Thelma Skare, Viviane Souza, Valéria Valim and Elaine Bezerra collaborated on this work, contributing to its conception, spreading the in-survey questionnaire, and data collection.

Authors' Contributions

Authors DCC, BSK, GAM, SEBD, JVZL, ARB, HDAM, KWPG, LDAV, NCA, SLER, AMK, APMGR, CM, ETR-N, EDSP, GSP, GAF, JRP, LMHM, RMX, MLMT, and MDMP have contributed to the study conception and protocol design, drafting and critically reviewing this manuscript. All authors have read and approved the final version of the manuscript.

Conflicts of Interest

None declared.

Multimedia Appendix 1

Survey instrument.

PDF File (Adobe PDF File), 67 KB

  1. Maia BR, Dias PC. Ansiedade, depressão e estresse em estudantes universitários: o impacto da COVID-19. Estudos de Psicologia (Campinas) 2020 May 18;37:1-8 [FREE Full text] [CrossRef]
  2. Brooks SK, Webster RK, Smith LE, Woodland L, Wessely S, Greenberg N, et al. The psychological impact of quarantine and how to reduce it: rapid review of the evidence. Lancet 2020 Mar 14;395(10227):912-920 [FREE Full text] [CrossRef] [Medline]
  3. Wilder-Smith A, Freedman DO. Isolation, quarantine, social distancing and community containment: pivotal role for old-style public health measures in the novel coronavirus (2019-nCoV) outbreak. J Travel Med 2020 Mar 13;27(2):taaa020 [FREE Full text] [CrossRef] [Medline]
  4. Meo SA, Abukhalaf AA, Alomar AA, Sattar K, Klonoff DC. COVID-19 pandemic: Impact of quarantine on medical students' mental wellbeing and learning behaviors. Pak J Med Sci 2020 May;36(COVID19-S4):S43-S48 [FREE Full text] [CrossRef] [Medline]
  5. Portaria Nº 492, de 23 de Março de 2020. Ministério da Saúde.   URL: [accessed 2021-03-24]
  6. Portaria Nº 374, de 3 de Abril de 2020. Ministério da Saúde.   URL: [accessed 2020-03-24]
  7. Chandratre S. Medical students and COVID-19: Challenges and supportive strategies. J Med Educ Curric Dev 2020 Jun 24;7:2382120520935059 [FREE Full text] [CrossRef] [Medline]
  8. Liu J, Zhu Q, Fan W, Makamure J, Zheng C, Wang J. Online mental health survey in a medical college in China during the COVID-19 outbreak. Front Psychiatry 2020 May 13;11:459 [FREE Full text] [CrossRef] [Medline]
  9. Moreira SDNT, Vasconcellos RLDSS, Heath N. Estresse na formação médica: como lidar com essa realidade? Rev Bras Educ Med 2015;39(4):558-564 [FREE Full text] [CrossRef]
  10. Meyer C, Guimarães ACDA, Machado Z, Parcias SR. Qualidade de vida e estresse ocupacional em estudantes de medicina. Rev Bras Educ Med 2012 Dec;36(4):489-498 [FREE Full text] [CrossRef]
  11. Gaughran F, Dineen S, Dineen M, Cole M, Daly RJ. Stress in medical students. Ir Med J 1997;90(5):184-185. [Medline]
  12. Azad N, Shahid A, Abbas N, Shaheen A, Munir N. Anxiety And depression in medical students of a private medical college. J Ayub Med Coll Abbottabad 2017;29(1):123-127 [FREE Full text] [Medline]
  13. Feodrippe ALO, Brandão MCDF, Valente TCDO. Qualidade de vida de estudantes de Medicina: uma revisão. Rev Bras Educ Med 2013 Sep;37(3):418-428 [FREE Full text] [CrossRef]
  14. Moutinho ILD, Maddalena NDCP, Roland RK, Lucchetti ALG, Tibiriçá SHC, Ezequiel ODS, et al. Depression, stress and anxiety in medical students: A cross-sectional comparison between students from different semesters. Rev Assoc Med Bras (1992) 2017 Jan 01;63(1):21-28 [FREE Full text] [CrossRef] [Medline]
  15. Estrela YDCA, Rezende ACC, Guedes AF, Pereira CDO, de Sousa MNA. Estresse e correlatos com características de saúde e sociodemográficas de estudantes de medicina. Estrés y características sociodemográficas y de salud en estudiantes de medicina. CES Medicina 2018 Dec;32(3):215-225 [FREE Full text] [CrossRef]
  16. Masic I, Naser N, Zildzic M. Public health aspects of COVID-19 infection with focus on cardiovascular diseases. Mater Sociomed 2020 Mar;32(1):71-76 [FREE Full text] [CrossRef] [Medline]
  17. Gomides A, Ferreira G, Kakehasi A, Lacerda M, Marques C, Mota L, et al. Impact of chronic use of antimalarials on SARS-CoV-2 infection in patients with immune-mediated rheumatic diseases: Protocol for a multicentric observational cohort study. JMIR Res Protoc 2020 Oct 14;9(10):e23532 [FREE Full text] [CrossRef] [Medline]
  18. Vignola RCB, Tucci AM. Adaptation and validation of the depression, anxiety and stress scale (DASS) to Brazilian Portuguese. J Affect Disord 2014 Feb;155:104-109. [CrossRef] [Medline]
  19. Lovibond SH, Lovibond PF. Manual for the Depression Anxiety Stress Scales (2nd Ed.). Sydney, Australia: Psychology Foundation of Australia; 1995.
  20. Eysenbach G. Improving the quality of web surveys: the Checklist for Reporting Results of Internet E-Surveys (CHERRIES). J Med Internet Res 2004 Sep 29;6(3):e34 [FREE Full text] [CrossRef] [Medline]
  21. Hennessy S, Bilker WB, Berlin JA, Strom BL. Factors influencing the optimal control-to-case ratio in matched case-control studies. Am J Epidemiol 1999 Jan 15;149(2):195-197. [CrossRef] [Medline]

CHERRIES: Checklist for Reporting Results of Internet E-Surveys
DASS: Depression, Anxiety, and Stress Scale
MPII: Mario Pinotti II
MERS: Middle Eastern Respiratory Syndrome
SARS: Severe Acute Respiratory Syndrome

Edited by G Eysenbach; submitted 28.09.20; peer-reviewed by E Oghogho, P Kanzow; comments to author 08.03.21; revised version received 11.03.21; accepted 17.03.21; published 09.04.21


©Débora Cerqueira Calderaro, Barbara Stadler Kahlow, Gabriela Araújo Munhoz, Samuel Elias Basualto Dias, João Vitor Ziroldo Lopes, Aline Rizzo Borges, Henrique De Ataíde Mariz, Kirla Wagner Poti Gomes, Lilian David De Azevedo Valadares, Nafice Costa Araújo, Sandra Lucia Euzébio Ribeiro, Adriana Maria Kakehasi, Ana Paula Monteiro Gomides Reis, Cláudia Marques, Edgard Torres Reis-Neto, Eduardo Dos Santos Paiva, Gecilmara Salviato Pileggi, Gilda Aparecida Ferreira, José Roberto Provenza, Licia Maria Henrique Mota, Ricardo Machado Xavier, Maycoln Leôni Martins Teodoro, Marcelo De Medeiros Pinheiro, The Voluntary Brazilian Society Of Rheumatology Task Force Against COVID-19. Originally published in JMIR Research Protocols (, 09.04.2021.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work, first published in JMIR Research Protocols, is properly cited. The complete bibliographic information, a link to the original publication on, as well as this copyright and license information must be included.