Published on 28.11.16 in Vol 5, No 4 (2016): Oct-Dec
Effect on Nitrogen Balance, Thermogenesis, Body Composition, Satiety, and Circulating Branched Chain Amino Acid Levels up to One Year after Surgery: Protocol of a Randomized Controlled Trial on Dietary Protein During Surgical Weight Loss
Background: Bariatric surgery (BS), the most effective treatment for severe obesity, typically results in 40-50 kg weight loss in the year following the surgery. Beyond its action on protein metabolism, dietary protein intake (PI) affects satiety, thermogenesis, energy efficiency, and body composition (BC). However, the required amount of PI after surgical weight loss is not known. The current daily PI recommendation for diet-induced weight loss is 0.8 g/kg ideal body weight (IBW) per day, but whether this amount is sufficient to preserve fat-free mass during active surgical weight loss is unknown.
Objective: To evaluate the effect of a 3-month dietary protein supplementation (PS) on nitrogen balance (NB), BC, energy expenditure, and satiety in women undergoing either gastric bypass or vertical sleeve gastrectomy.
Methods: In this randomized prospective study, participants will be randomized to a high protein supplementation group (1.2 g/kg IBW per day) or standard protein supplementation group (0.8 g/kg IBW per day) based on current guidelines. Outcome measures including NB, BC, circulating branched chain amino acids, and satiety, which will be assessed presurgery, and at 3-months and 12-months postsurgery.
Results: To date, no studies have examined the effect of dietary PS after BS. Current guidelines for PI after surgery are based on weak evidence.
Conclusions: The results of this study will contribute to the development of evidence-based data regarding the safe and optimal dietary PI and supplementation after BS.
Trial Registration: Clinicaltrials.gov NCT02269410; http://clinicaltrials.gov/ct2/show/NCT02269410 (Archived by WebCite at http://www.webcitation.org/6m2f2QLeg).
JMIR Res Protoc 2016;5(4):e220
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Bariatric surgery (BS) is the most effective long-term therapy for the treatment of severe obesity. BS is associated with a favorable impact on overall and cardiovascular mortality, incidence of first occurrence of fatal or nonfatal cardiovascular events, prevention and remission of type 2 diabetes mellitus (T2DM), and quality of life . Short-term studies showed no apparent difference between gastric bypass (GBP) and vertical sleeve gastrectomy (SG) on T2DM remission and weight loss [ ]. GBP and SG are the most accepted procedures currently being performed, with SG increasing in prevalence since its inception in 2003 [ ]. Although the surgeries differ widely (GBP is considered a malabsorptive and restrictive procedure while SG is solely restrictive), the prevalence of nutrient deficiencies seems to be comparable [ ]. However, the metabolic impact of dietary protein intake (PI) in the early phase of active weight loss has not been studied.
PI during diet-induced weight loss and weight maintenance has been associated with retention of fat-free mass (FFM) [, ], better satiety [ ], and, if insufficient, with malnutrition [ ]. Surgical weight loss is associated with decreased circulating levels of branched chain amino acids (BCAAs) [ ].
Caloric intake decreases significantly during the first 3-6 months after surgery and may be frequently associated with vitamin, mineral [, ], and protein deficiencies [ , - ]. Prospective studies observed that low albumin levels, a clinical marker of protein deficiency [ ], can occur up to 2 years after GBP [ , ] with a prevalence ranging from 3 to 18% [ , - ]. Protein deficiency is more commonly observed after malabsorptive procedures, such as the biliopancreatic diversion [ ]. Changes in taste and food preferences, and some degree of stomach discomfort during meals (with or without dumping syndrome), contribute to a poor dietary protein tolerance, thereby affecting the net PI [ ]. The potential macronutrient maldigestion and/or malabsorption observed after BS [ ] may also contribute to a compromised protein status.
It is generally accepted that diets containing all indispensable amino acids (AAs) are required for optimal protein synthesis and balance [, ], and optimal intake of dietary protein should even be increased in vulnerable populations [ ]. Nitrogen balance (NB), the difference between nitrogen intake and loss, is often compromised with trauma or infection, even with attempted nutritional interventions [ , ]. BS compromises NB via lower PI and an early maintained (or generally higher) protein demand following surgery, so high protein diets are recommended by various guidelines [ - ]. The most updated American Association of Clinical Endocrinologists guidelines suggest that a minimal PI of 60 g/day (and up to 1.5 g/kg ideal body weight [IBW] per day) after BS should be adequate, although these recommendations are only supported by a low level of scientific evidence (grade C or D). However, as the clinical tolerance of protein-containing foods is low after BS, recommendations are rarely followed and patients often do not reach their PI goal [ ]. Our group and others have shown that daily consumption of 60 g of protein can be challenging during the first 4 months after surgery, even when protein supplements are recommended and supplied at no cost [ ]. PI of 1.5 g/kg IBW per day would represent (when considering IBW for a body mass index [BMI] of 25 kg/m2) 105 g/day for a woman with an IBW of 70 kg. The low protein tolerance mentioned above makes this recommendation difficult to follow, even with the most motivated patients. Therefore, finding an acceptable amount of PI would ensure optimal FFM retention, limit muscle breakdown, maintain resting energy expenditure (REE), and contribute to the development of a healthier diet that supports weight loss maintenance without interfering with glucose homeostasis.
Risk of Decreased Lean Body Mass and Resting Energy Expenditure With Surgical Weight Loss: Effect of Dietary Protein
The consequences of negative energy and protein balance on visceral mass and skeletal muscle mass are well established . Surgical weight loss results in both fat mass (FM) and lean body mass (LBM) loss: 75.2% and 24.8%, respectively [ ]. LBM is the main determinant of REE, and explains 70% of the REE variance [ ], with REE being the largest component of 24-hour energy expenditure (EE). Thus, reduced EE after weight loss is a factor of resistance for weight loss, and it may trigger the regaining of weight in the BS population [ ]. The impact of daily PI on REE after BS has not been previously addressed, while PI is known to impact postprandial thermogenesis. Although the reduction of FM in obese individuals during weight loss is beneficial, the decrease in LBM may down-regulate the metabolic process, including protein turnover and basal metabolic rate, thus compromising long-term healthy weight management [ ]. Studies on the impact of PI on body composition (BC) after BS are scarce and inconclusive. While some studies failed to find a significant correlation between absolute PI and FFM loss relative to total weight loss after BS [ , - ], others found that higher levels of PI improved BC changes by enhancing the loss of FM and reducing FFM loss after BS [ ]. High protein diets may increase EE while preventing LBM loss [ ] during weight loss. Increased EE from dietary protein is attributed to an enhanced thermic effect of food (15%, standard deviation [SD] 4) compared to carbohydrates (6%, SD 2) or lipids (7%, SD 3) [ ]. Studies related to the effect of high protein versus standard protein diets on the prevention of LBM loss, which in turn lead to a lesser reduction in REE, are often inconclusive [ , , ]. In addition, comparisons between GBP and SG have not been completed.
Nitrogen Balance Study in the Bariatric Surgery Setting
NB is classically used to determine adequate PI with regards to daily nitrogen loss, and to estimate whole body protein balance in response to nutritional interventions . Sustained negative NB can be associated with loss of lean and fat tissue [ ]. Thus, ideally the goal of PI after BS should aim at preventing and/or limiting negative NB, even under energy restriction.
Occurrence of malabsorption should be considered when assessing NB in BS subjects . In a malabsorptive state, fecal losses of nitrogen may be as high as 3.5 g/day [ ]. Thus, in addition to the other components of the NB equation, fecal nitrogen losses should be measured after BS, and not simply estimated at 0.4 g/day, as recommended by the Joint Food and Agriculture Organization/World Health Organization expert committee, under nonmalabsorptive conditions [ , ]. Of note, Odstrcil et al [ ] studied the contribution of malabsorption on the reduction in net energy absorption 5 and 14 months after long-limb GBP. Net absorption of protein was significantly reduced after BS, and malabsorption accounted for 13% of the total reduction in protein absorption at both study time points [ ]. However, a protein kinetic study using stable isotopes demonstrated that protein digestion and absorption were not impaired, and even accelerated, 3 months after GBP [ ].
Roles of Protein Supplementation on Circulating Levels of Amino Acids
It has long been recognized that circulating levels of AAs, including BCAAs, are elevated in persons with obesity, insulin resistance (IR), or T2DM, compared to healthy controls [, ]. BS is associated with reduced concentrations of plasma BCAAs [ ] and decreased IR [ , ]. To date, the protein sparing effect of long-term protein supplementation (PS) has not been studied.
Dietary Protein Intake and Satiety
High PI has been shown to increase satiety in the context of energy restriction [, ]. Proposed factors that may enhance satiety include: a ketogenic state; relatively elevated plasma AA levels [ ]; an increase of the satiety peptide YY (PYY), glucagon-like peptide 1 (GLP-1) and cholecystokinin (CCK) [ ]; and/or a decrease of the orexigenic hormone ghrelin [ , ]. We aim to further explore the relationship between PI and satiety after BS procedures.
Considering the complexity of metabolic and behavioral changes after BS, the overall aim of our research study will be to establish adequate PI after BS. To achieve this goal, we will compare the effect of 2 levels of PI (high protein supplementation group, HPS-G; and standard protein supplementation group, SPS-G) after BS (GBP and SG) on (1) NB, (2) BC, (3) REE and diet-induced thermogenesis (DIT), (4) satiety, (5) the release of gut hormones, (6) circulating levels of BCAAs in relation to insulin sensitivity, and (7) adherence to protein supplements. Five specific objectives will address our aims.
Objective 1: Nitrogen Balance
Total body NB will be measured to assess the levels of PI and protein absorption. The measure of NB will be performed during an inpatient stay before surgery, after 3 months of controlled PS, and 12 months after BS. PI will be established at 1.2 g protein/IBW per day for all participants in the month before surgery. After surgery, participants will be randomized to either 1.2 g protein/IBW per day (HPS-G) or 0.8 g protein/IBW per day (SPS-G). Participants will receive PS for 3 months after BS, up to the second inpatient study time point. During the inpatient stay, all foods and drinks will be strictly controlled, and 24-hour urine and stool samples will be collected. Nitrogen content of food from aliquots, urine, and stool will be measured, as explained in the Methods section.
Objective 2: Effect of Protein Supplementation on Body Composition
We will compare the effect of HPS and SPS on LBM. Changes in BC will be assessed before surgery, at 3 months, and 12 months after surgery in the 2 PS study groups using anthropometry, total body water (TBW), and plethysmography (BOD POD).
Objective 3: Effect of Protein Supplementation on Resting Energy Expenditure and Diet-Induced Thermogenesis
We will compare the effect of HPS and SPS on EE, measured by indirect calorimetry at rest (REE) and 4 hours after a high protein liquid test meal (DIT), before surgery, at 3 months, and 12 months after BS.
Objective 4: Branched Chain Amino Acid Levels
We will characterize changes in circulating BCAA levels in relation to insulin sensitivity and PI adequacy after GBP and SG. Circulating BCAA levels will be measured by targeted metabolomics and compared to insulin sensitivity (calculated by the homeostatic model assessment-insulin resistance or Matsuda index) before surgery, at 3 months, and 12 months postsurgery.
Objective 5: Effect of Surgical Procedures on Nitrogen Balance and Satiety
We will compare the effect of HPS and SPS, the hormonal response after a meal, and NB between GBP and SG. Satiety and hunger will be measured by visual analog scales (VASs) while fasting and in response to a high protein meal, before surgery, at 3 months, and 12 months after BS. Blood samples will be obtained before and after the meal test to measure the satiety-related gut hormones CCK, PYY, GLP-1, ghrelin, along with insulin and glucose levels. NB will also be compared between the surgical procedures.
All subjects will be recruited from the Bariatric Surgery Institute at Mount Sinai St Luke’s Hospital (New York, NY), and will be required to sign an institutional review board -approved consent form prior to enrollment.
Number of Subjects
A total of 112 volunteers scheduled to undergo either GBP or SG will be recruited. Based on our experience, we anticipate a conservative 30% attrition rate. Therefore, approximately 80 patients are expected to remain in the study at completion.
Inclusion and Exclusion Criteria
BC differences exist between women and men, and the BS patient population is 75% women, so only premenopausal women (18-40 years of age, BMI <50 kg/m2) will be included in this study. Other inclusion criteria include: any race/ethnicity; patients scheduled to undergo either GBP or SG; treated or untreated resting systolic/diastolic blood pressure less than 160/100 mmHg; fasting triglyceride concentration less than 600 mg/dl, without regard for diagnosis or prescription for other dyslipidemias; absence of diabetes, or diet-controlled diabetes (taking no medications).
Exclusion criteria include: presence of nitrogen retention disease (eg, renal or hepatic disease); abnormal thyroid function; known malabsorption syndrome; cardiovascular disease; current mucosal (gastrointestinal, respiratory, urogenital) or skin (cellulitis) infection; any psychiatric disorder; and any other condition which, in the opinion of the investigators, may make the candidate unsuitable for participation in this study.
Design and Setting
This study is a prospective randomized controlled trial (RCT), in which 112 obese participants (with no major comorbidities) scheduled to undergo GBP or SG will be randomly allocated to SPS-G or HPS-G cohorts. Participants will undergo three 5-day inpatient stays: presurgery, 3 months postsurgery, and 12 months postsurgery. The inpatient stays will be in the Clinical Research Resource (CRR) in the Irving Institute of Clinical and Translational Research at Columbia University Medical Center. The overall study design is displayed in.
Protein Supplementation Protocol
PS regimens will be supplied during the 3 months immediately following surgery. The HPS-G group will receive 1.2 g protein/kg IBW per day, and the SPS-G group will receive 0.8 g protein/IBW per day, with IBW established as a BMI of 25 kg/m2 . PS goals will be achieved partly by providing subjects with an instant high quality protein-isolate whey protein powder-supplement (Unjury; ProSynthesis Laboratories, Sterling, VA) [ ]. Whey protein is the richest source of BCAAs and leucine, which has been demonstrated to stimulate muscle protein synthesis in elderly populations [ , ]. The SPS-G group will require approximately 1.5 servings per day (21 g protein/serving) while the HPS-G group will require 3 servings per day to meet their individually prescribed protein requirement.
Preceding Diet at Baseline
The NB study will be preceded by an outpatient diet stabilization period lasting 7 days. During this time, a meal plan will be tailored to each participant and PS will be provided, based on the individual’s preferences, to promote adherence. Participants will be asked to complete a 7-day food record during this outpatient period, and will be contacted by phone one time during this period in order to evaluate adherence to, and tolerance of, the prescribed diet. This diet stabilization period is designed to allow each participant to adapt to the required level of PS . Energy and protein requirements will be based on 35 kilocalorie (kcal)/IBW per day and 1.2 g protein/IBW per day, respectively [ ], and will be calculated using the Nutrition Data System for Research (NDSR), 2011 [ ]. Energy intake will be adjusted to minimize weight changes (within 0.75 kg of body weight) during the period of controlled diet, prior to admission, and during the inpatient stay [ ].
Inpatient Study Period Before Surgery
During the initial 5-day inpatient study period all foods and drinks will be provided, and carefully monitored and controlled, under the supervision of the research dietician (VM) and the staff of the Bionutrition Unit in the CRR. All foods, drinks, and protein supplements will be aliquoted and weighed on a 0.1-gram precision scale before consumption. To minimize variability and accurately control the intake of nitrogen, participants will eat the same diet every day. Participants will not be allowed to eat or drink any other food except for tap-filtered water. Diets will be based on regular/natural foods that require minimum manipulation. To meet the dietary PI goals, Unjury isolate whey protein products will be included in the diet as the main source of protein during the entire 5-day period. Vitamin and mineral supplementation, if any, will be continued during the inpatient study. Participants will be asked to eat the entire food portion that is served. Supervision will be ensured during each meal, and after each serving the trays will be examined by staff members, and the volume of any unconsumed beverage/soup will be measured in a graduated cylinder. All existing uneaten food will be weighed and recorded for day-to-day dietary adjustments. Meals for 1 complete day during the stay will be prepared and cooked in duplicate, and will be homogenized in a heavy duty commercial blender to obtain 24-hour diet aliquots. Two aliquots will be frozen at –70° Celsius for later analysis of total nitrogen and energy content.
Post-Bariatric Surgery Dietary Protein Supplementation Intervention
After BS, participants will be randomized using the technique of permuted block randomization to ensure that equal numbers of patients are assigned to each treatment arm (HPS-G or SPS-G). In accordance with post-BS practice guidelines, the meal plan will consist of 6 small meals per day (breakfast, morning snack, lunch, afternoon snack, dinner, and late snack), plus powdered PS that will be distributed during either 3 (SPS-G) or 6 (HPS-G) meals. Phone and in-person contacts will be provided on alternating weeks during the 12-week PS intervention. At each contact time point, dietary and PS adherence, food tolerance, and hydration will be evaluated by reviewing the food records specifically designed to quantify the number of scoops of the PS used in each meal. Urinary nitrogen excretion will be used as a biomarker for PI, and to measure compliance. Subjects will be asked to collect their 24-hour urine at 5 different time points during the 12-week period. PS containers will be supplied during the in-person participant-dietitian biweekly interviews. Participants will be asked to bring the empty PS containers for review and quantification at each appointment.
Preceding Diet and 5-Day Inpatient Stays at 3 Months and 12 Months After Bariatric Surgery
Daily energy intake during the preceding diet period, and during the inpatient stays, will be significantly reduced to a 10-15 kcal/IBW per day at 3 months post-BS. This value will increase up to 20 kcal/day at 12 months .
Laboratory Analyses and Outcome Measurements
Nitrogen Balance Study
Participants will be admitted for 5 days in the CRR for complete feces and urine collections during the last 3 days of the inpatient period. These collections will take place at the 3 different study time points: (1) presurgery; (2) 3 months after BS, during the active weight loss phase; and (3) 12 months after BS to evaluate the long-term carry over effect of PS on BC changes and REE.
24-Hour Complete Specimens Collection
Four 24-hour urine collections will be undertaken; 1 per day during the inpatient stay. Collection will start after the first void of the second day. Labeled urine containers will be refrigerated at 4° Celsius. Starting on the third day, and until the end of the inpatient study period, all stools will be collected with a specimen container kit placed in the toilet. Samples will be processed every day and aliquots will be obtained and stored at –70° Celsius for later analysis of 24-hour nitrogen content. To ensure a complete collection of feces and compliance with the inpatient diet, 1.5 g of polyethylene glycol (PEG) will be ingested along with other food items during the first inpatient day. PEG has been used as a fecal marker to follow time and completeness of collections, to determine when experimental diets have been eliminated, and to correct for differences in the day-to-day variation of fecal transit time [, ]. Stool samples will be analyzed for PEG, and combustible energy from PEG will be subtracted from fecal combustible energy measured by bomb calorimetry.
Miscellaneous losses of nitrogen in sweat, sloughed skin, nails, hair, and various bodily secretions will be estimated at 8 mg/kg of body weight per day . Body weight will be measured daily during the inpatient stays to estimate the daily miscellaneous nitrogen losses defined above.
NB will be calculated using the difference between daily nitrogen intake and total nitrogen losses as follows: nitrogen balance = dietary nitrogen intake–(daily urinary nitrogen excretion + daily fecal nitrogen excretion + daily miscellaneous nitrogen losses).
Anthropometry and Body Composition
Anthropometry will be assessed during admission at the CRR before BS, at 3 months, and 12 months after surgery. Participants will be weighed while wearing a hospital gown, and without shoes, to the nearest 0.1 kg. Height will be determined using a fixed wall stadiometer to the nearest 0.1 cm. BMI will be calculated as weight (kg) divided by the square of the height (meters).
BC will be estimated based on a 3-compartment model, using 3 independent measures: body weight, TBW, and body density. To determine changes in BC after BS-induced weight loss, the 3-compartment model will be used .
Total Body Water
TBW will be measured using the stable isotope deuterium oxide (D2 O) as the reference method. A baseline venous blood sample (approximately 7 mL) from an antecubital vein will be taken while fasting. Immediately afterwards, a known dose of deuterated water D2 O (1 g/kg) will be taken orally. Three hours following the dose, when the D2 O has equilibrated with the body water deuterium-to-hydrogen, a second fasting blood specimen will be taken (approximately 7 mL). The TBW will then be derived from the increase in plasma deuterium content in relation to the volume of D2 O ingested .
Air Displacement Plethysmography
Body density will be measured using the BOD POD (Cosmed, Chicago, IL; software version 2.3) [, ]. Subjects will be clothed in a Lycra-style swim cap and tight fitting underwear. Body weight will be measured to the nearest 1 g on the BOD POD electronic weight scale. Following standard calibration procedures, the subject’s body volume will be measured, with correction made for thoracic gas volume, which will be estimated using the BOD POD breathing circuit system. The final thoracic gas volume and the average of 2 body volume measurements within 0.2% will be used to calculate body density [ ].
FFM and FM will be measured using a modified 3-compartment model that was developed for obese subjects in the New York Obesity Research Center Body Composition laboratory, as follows: fat (kg) = 2.122 × (body weight/density) – 0.779 × TBW – 1.356 × body weight . Body weight is measured in kg (measured by the Weight Tronix scale), density is derived from BOD POD, and TBW is measured in kg. FFM will be calculated as body weight minus FM.
EE will be measured during the last day of the 3 inpatient stays (presurgery, 3 months, and 12 months postsurgery). Height, weight, blood pressure, pulse, and temperature measurements will be taken, and an intravenous catheter will be inserted. At 8:00 a.m., subjects will be placed under the hood of the metabolic cart (Parvo Medics System, True Max 2400)  and rest for 30 minutes. Following the resting period, REE will be measured for 30 minutes and baseline blood samples will be taken. Subsequently, all subjects will consume an isocaloric liquid meal (Boost High Protein) over 10 minutes. Subjects will receive acetaminophen with the meal to measure gastric emptying. Following meal administration, DIT (amount of EE above the REE rate due to the cost of processing food for use and storage) will be measured for 4 hours. Blood samples and questionnaire measurements will be collected at –15, 0, 15, 30, 60, 90, 120, and 180 minutes to measure hormonal and perceived satiety. REE will be calculated as an average of the last 15-20 minutes of each measurement period if values have reached steady state, defined as <10% fluctuation in minute ventilation and oxygen consumption and <5% fluctuation in respiratory quotient. DIT will be calculated by measuring the area under the curve of postprandial metabolic rate, above extrapolated baseline REE, for each time period [ ]. Baseline and postprandial CCK, GLP-1, ghrelin, and PYY gut peptide concentrations will also be measured during the meal test.
Subjective and objective assessments of satiety will be collected before the surgery, at 3 months, and at 12 months postsurgery, during the meal test on the last day of the inpatient stay, using 2 different approaches: VASs, and measurements of hormonal signals of hunger and satiety.
Visual Analog Scales
Participants will rate their feelings on the following questions by means of a mark on 100-mm line VASs: “How hungry are you?”, “How full are you?”, “How much stomach discomfort do you feel?”, “How thirsty are you?”, and “How much anxiety and nervousness do you feel?” The scale will be anchored at the low end with the lowest intensity feelings (eg, not at all), and with opposing terms at the high end (eg, most imaginable), as previously described in the literature .
Measurements of Hormonal Signals of Satiety
Subjects will be instructed to consume the test meal within 15 minutes. Calorie intake and nutrient distribution of the meal (Boost High Protein) will be as follows: calories, 240; protein, 15 g; carbohydrates, 33 g; fat, 6 g; sodium, 200 mg; potassium, 400 mg, fiber 0 g. Patients will be able to choose between vanilla and chocolate flavors., An intravenous catheter will be inserted at 7:00 a.m. on the day of the experiment and blood will be drawn at before the meal, and at 15, 30, 60, 90, 120, and 180 minutes after the meal to measure hormonal signals of satiety. Blood samples will be collected in ethylenediaminetetraacetic acid tubes with added aprotinin (500 kallekrein inhibitory units per mL of blood) and dipeptidyl peptidase-4 inhibitor (10 μl/mL of blood; Millipore Research), and stored at –70° Celsius. Plasma concentrations of PYY, CCK, GLP-1, ghrelin, and insulin will be determined by radioimmune assay, and glucose concentration will be determined using the glucose oxidase method with an Analox glucose analyzer (Analox Instruments, Lunenburg, MA). Serum acetaminophen levels will be measured using an enzyme-linked immunosorbent assay (Abbot Laboratories, Chicago, IL).
Measure of Food Reward
Food reward is considered a strong eating drive that could override satiety, and will be assessed by the reward-based eating drive scale . This 9-question screening tool will be completed by the participant during the inpatient stay before surgery, at 3 months, and at 12 months postsurgery.
Metabolomics—Branched Chain Amino Acids
Fasted blood samples obtained prior to the meal test will be used to measure circulating BCAAs by mass spectrometry, as previously described . BCAAs will be measured during the inpatient stay before surgery, at 3 months, and at 12 months postsurgery.
Insulin sensitivity will be measured by the Matsuda index during the meal test as follows: 10,000/(fasting glucose × fasting insulin × mean glucose from from 0 to 180 min) × mean insulin from from 0 to 180 min)^0.5 .
There will be no physical activity intervention in this study. A measure of the free-living physical activity will be obtained using the ActiGraph, a wireless activity monitor that will provide 168 continuous hours (1 week) of measurement before surgery, at 3 months, and at 12 months postsurgery.
Vitamins, Minerals, Prealbumin, and Albumin Levels
Laboratory assessments will be obtained as part of the regular blood tests taken before surgery, at 3 months, and at 12 months postsurgery, following the clinical practice guidelines for the evaluation of the nutritional status in the Bariatric Clinic.
Dietary intake evaluation during the outpatient phase of the study will be performed as part of each nutritional follow-up. Food and beverage intake will be assessed using either a 7-day (during the preceding diet and the inpatient stay) or 4-day (regular dietary evaluations) throughout the study. As described above, the PI goal will be accomplished for each group (HPS-G and SPS-G) by using the specific PS resources provided. During the screening phase of the study, all subjects will attend 2 training sessions delivered by a Registered Dietitian, in which they will be instructed on how to record their food intake and include at least 1 weekend day when recording. This information will be analyzed with NDSR.
Sample Size Calculations
Sample size was calculated based on anticipated changes in REE during weight loss using data from published literature  and changes in BCAAs from our previous study [ ]. Baba et al [ ] studied changes in REE after high protein (n=7) versus low protein (n=6) weight loss diets. Weight loss was significantly higher in the high protein group (8.3 kg, SD 0.7) compared to the low protein (6.0 kg, SD 0.6). Change in REE was –132.3 kcal/day (SD 51.0) in the high protein group and –384.3 kcal/day (SD 84.6) in the low protein group. Assuming a Cronbach alpha of 0.05 and 0.80 (80%) power we would need to recruit 16 participants to each study group. Laferrère et al [ ] showed that for a matched amount of weight loss (10 kg) in GBP (n=10) versus diet-induced weight loss (n=11), BCAA changes were –176.4 (SD 96.6) and –57.6 (SD 99.3) in surgical and nonsurgical groups, respectively. Assuming a Cronbach alpha of 0.05 and 0.80 (80%) power we would need to recruit 22 participants to each study group. Therefore, accounting for an attrition rate of 30% after 1 year, we will enroll a total of 28 subjects per group.
Data will be analyzed using the SPSS statistical program (IBM Software; Armonk, NY). For most study aims, a repeated measures design will be used to examine the trajectory of changes in subjects between HPS-G and SPS-G groups regarding BC, EE, NB, satiety, and circulating BCAAs, from presurgery to 3-month and 12-month postsurgery levels. Nonlinear mixed model regression (SAS PROC NLMIXED) will be used for the actual analyses since these outcomes are not likely to be normally distributed. In addition to overall tests for differences between groups (GBP and SG, and HPS-G vs SPS-G), differences over time (presurgery, 3 months, and 12 months postsurgery), and group per time interactions. Other factors will be explored as covariates to determine possible explanatory factors for significant differences between presurgery and 3-month and 12-month postsurgery levels. Significance levels will be adjusted based on the total number of comparisons being carried out, using a Bonferroni correction. Secondary outcomes will be analyzed using a similar repeated measures design with either mixed model regression or nonlinear mixed model regression where appropriate. No adjustment for the number of comparisons will be made in the case of the secondary outcomes (ie, Cronbach alpha will be 0.05 for all comparisons).
This proposal has been approved by the Institutional Review Board of Mount Sinai St Luke’s Hospital and Columbia University. Voluntary written informed consent will be obtained from each participant prior to enrollment.
The proposed study will determine the effect of 2 different levels of dietary PI (HPS-G and SPS-G) after SG and GBP on the NB, BC, EE, hormonal and perceived satiety, plasma levels of BCAAs, and insulin sensitivity, and feasibility of PS up to 1 year after BS. In addition, the analysis of energy excreted in feces will aid in the understanding of how much malabsorption exists in the 2 procedures studied (GBP and SG). Satiety during a liquid test meal will be assessed and its possible mediators, gastrointestinal hormones released, and gastric emptying rate will also be determined.
Two nonrandomized studies demonstrated that higher levels of PI (>60 g/day) were related to higher excess weight loss 6 months after BS  and at 3, 6, and 12 months postsurgery [ ]. Other studies have failed to observe a significant association between PI and excess weight loss [ , , ]. PI was positively associated with LBM retention in 3 nonrandomized studies [ , , ], although this association was not found in other studies up to 1 year after BS [ , ]. The same authors also failed to observe a relationship between PI and REE [ , ]. The relationship between PI levels and gastrointestinal hormones needs to be explored more thoroughly. One RCT failed to observe a relationship between PI and GLP-1 or ghrelin [ ]. The relationship between BCAA circulation levels and glucose homeostasis after BS also needs further attention. Elevated circulating BCAAs are associated with obesity and T2DM. Comprehensive metabolic profiling of obese versus lean human subjects revealed a BCAA metabolic signature, marked by increased circulating levels of BCAAs as well as products of BCAA catabolism [ ]. The reason that circulating BCAA levels are elevated in obesity is still unclear. The mechanisms responsible for the decrease in BCAA serum levels with weight loss [ ] or BS [ , , ] are still being studied. Supplementation of a high fat diet with BCAAs in rats [ ], or infusion of AAs in humans [ ], results in IR. A recent epidemiological study reported that elevated plasma levels of essential AAs, including BCAAs, phenylalanine, and tyrosine in healthy individuals predicted a 5-fold increase in the risk of developing T2DM [ ]. To our knowledge, there are no intervention studies that address the impact of PI on BCAA serum levels after GBP or SG. Lower levels of PI seem to have a positive effect on glucose homeostasis, while sustained low circulating levels of BCAAs may have a negative impact on protein synthesis and the integrity of the skeletal muscle mass during weight loss [ ]. Plasma leucine concentration has been shown to correlate with skeletal muscle protein synthesis [ ], so the metabolomic study of BCAAs during the high protein meal test will contribute to the study of AA kinetics after massive weight loss induced by BS. Measuring the NB during a negative energy balance will provide an important means of understanding absorption and bioavailability of nitrogen.
Dietary guidelines, including PS after BS, are still under discussion since the levels of evidence of their recommendations are C or D . As previously detailed, the relationship between dietary PI and the various outcome variables that will be measured in this proposed study are not well established, and the available literature is contradictory. Dietary protein plays an important role in weight loss and obesity-related comorbidities, such as diabetes [ ]. BS is highly popular, making the proposed work relevant, and the study will help to clarify the relationship between PI and BS outcomes by addressing some of the existing gaps in the scientific literature.
This project is supported by the National Center for Advancing Translational Sciences, and the National Institutes of Health (Grant Number UL1 TR000040), formerly the National Center for Research Resources (Grant Number UL1 RR024156). VM is funded by the Marie Curie International Outgoing Fellowships for Career Development (FP7- PEOPLE-2012-IOF, Proposal #326342).
VM and PM drafted the manuscript. BL, XP, JV, and YB participated in designing the study and provided manuscript revisions. All authors read and approved the final manuscript.
Conflicts of Interest
- Sjöström L. Review of the key results from the Swedish Obese Subjects (SOS) trial - a prospective controlled intervention study of bariatric surgery. J Intern Med 2013 Mar;273(3):219-234 [FREE Full text] [CrossRef] [Medline]
- Jiménez A, Casamitjana R, Flores L, Viaplana J, Corcelles R, Lacy A, et al. Long-term effects of sleeve gastrectomy and Roux-en-Y gastric bypass surgery on type 2 diabetes mellitus in morbidly obese subjects. Ann Surg 2012 Dec;256(6):1023-1029. [CrossRef] [Medline]
- Angrisani L, Santonicola A, Iovino P, Formisano G, Buchwald H, Scopinaro N. Bariatric surgery worldwide 2013. Obes Surg 2015 Oct;25(10):1822-1832. [CrossRef] [Medline]
- Moizé V, Andreu A, Flores L, Torres F, Ibarzabal A, Delgado S, et al. Long-term dietary intake and nutritional deficiencies following sleeve gastrectomy or Roux-En-Y gastric bypass in a mediterranean population. J Acad Nutr Diet 2013 Mar;113(3):400-410. [CrossRef] [Medline]
- Moizé V, Andreu A, Rodríguez L, Flores L, Ibarzabal A, Lacy A, et al. Protein intake and lean tissue mass retention following bariatric surgery. Clin Nutr 2013 Aug;32(4):550-555. [CrossRef] [Medline]
- Abdeen G, le Roux CW. Mechanism underlying the weight loss and complications of Roux-en-Y gastric bypass. Review. Obes Surg 2016 Feb;26(2):410-421 [FREE Full text] [CrossRef] [Medline]
- Morínigo R, Moizé V, Musri M, Lacy AM, Navarro S, Marín JL, et al. Glucagon-like peptide-1, peptide YY, hunger, and satiety after gastric bypass surgery in morbidly obese subjects. J Clin Endocrinol Metab 2006 May;91(5):1735-1740. [CrossRef] [Medline]
- Brolin RE, LaMarca LB, Kenler HA, Cody RP. Malabsorptive gastric bypass in patients with superobesity. J Gastrointest Surg 2002;6(2):195-203; discussion 204. [Medline]
- Magkos F, Bradley D, Schweitzer G, Finck B, Eagon J, Ilkayeva O, et al. Effect of Roux-en-Y gastric bypass and laparoscopic adjustable gastric banding on branched-chain amino acid metabolism. Diabetes 2013 Aug;62(8):2757-2761 [FREE Full text] [CrossRef] [Medline]
- Alexandrou A, Armeni E, Kouskouni E, Tsoka E, Diamantis T, Lambrinoudaki I. Cross-sectional long-term micronutrient deficiencies after sleeve gastrectomy versus Roux-en-Y gastric bypass: a pilot study. Surg Obes Relat Dis 2014;10(2):262-268. [CrossRef] [Medline]
- Brolin RE, Kenler HA, Gorman JH, Cody RP. Long-limb gastric bypass in the superobese. A prospective randomized study. Ann Surg 1992 Apr;215(4):387-395. [Medline]
- Gibson R. Principles of nutritional assessment, 2nd ed. New York: Oxford University Press; 2005.
- Skroubis G, Sakellaropoulos G, Pouggouras K, Mead N, Nikiforidis G, Kalfarentzos F. Comparison of nutritional deficiencies after Roux-en-Y gastric bypass and after biliopancreatic diversion with Roux-en-Y gastric bypass. Obes Surg 2002 Aug;12(4):551-558. [CrossRef] [Medline]
- Byrne TK. Complications of surgery for obesity. Surg Clin North Am 2001 Oct;81(5):1181-1193. [Medline]
- Stocker DJ. Management of the bariatric surgery patient. Endocrinol Metab Clin North Am 2003 Jun;32(2):437-457. [Medline]
- Dolan K, Hatzifotis M, Newbury L, Lowe N, Fielding G. A clinical and nutritional comparison of biliopancreatic diversion with and without duodenal switch. Ann Surg 2004 Jul;240(1):51-56. [Medline]
- Moize V, Geliebter A, Gluck ME, Yahav E, Lorence M, Colarusso T, et al. Obese patients have inadequate protein intake related to protein intolerance up to 1 year following Roux-en-Y gastric bypass. Obes Surg 2003 Feb;13(1):23-28. [CrossRef] [Medline]
- Odstrcil EA, Martinez JG, Santa Ana CA, Xue B, Schneider RE, Steffer KJ, et al. The contribution of malabsorption to the reduction in net energy absorption after long-limb Roux-en-Y gastric bypass. Am J Clin Nutr 2010 Oct;92(4):704-713 [FREE Full text] [CrossRef] [Medline]
- World Health Organization, Food and Agriculture Organization of the United Nations, United Nations University. Protein and amino acid requirements in human nutrition: Report of a joint FAO/WHO/UNU expert consultation (WHO Technical Report Series 935). 2012 Jun 16. URL: http://apps.who.int/iris/bitstream/10665/43411/1/WHO_TRS_935_eng.pdf?ua=1 [accessed 2016-11-10] [WebCite Cache]
- Panel on Dietetic Products, Nutrition and Allergies. Scientific opinion on dietary reference values for protein. EFSA Journal 2012 Feb 09;10(2):2557. [CrossRef]
- Bauer J, Biolo G, Cederholm T, Cesari M, Cruz-Jentoft A, Morley J, et al. Evidence-based recommendations for optimal dietary protein intake in older people: a position paper from the PROT-AGE Study Group. J Am Med Dir Assoc 2013 Aug;14(8):542-559 [FREE Full text] [CrossRef] [Medline]
- O'Keefe SJ, Sender PM, James WP. “Catabolic” loss of body nitrogen in response to surgery. Lancet 1974 Nov 2;2(7888):1035-1038. [Medline]
- Cuthbertson D. Observations on the disturbance of metabolism produced by injury to the limbs. Q J Med 1932 Apr 1;1(2):233-246.
- Mechanick J, Youdim A, Jones D, Garvey W, Hurley D, McMahon M, American Association of Clinical Endocrinologists, Obesity Society, American Society for Metabolic & Bariatric Surgery. Clinical practice guidelines for the perioperative nutritional, metabolic, and nonsurgical support of the bariatric surgery patient--2013 update: cosponsored by American Association of Clinical Endocrinologists, the Obesity Society, and American Society for Metabolic & Bariatric Surgery. Endocr Pract 2013;19(2):337-372 [FREE Full text] [CrossRef] [Medline]
- Fried M, Yumuk V, Oppert J, Scopinaro N, Torres A, Weiner R, International Federation for Surgery of Obesity and Metabolic Disorders-European Chapter (IFSO-EC), European Association for the Study of Obesity (EASO), European Association for the Study of Obesity Obesity Management Task Force (EASO OMTF). Interdisciplinary European guidelines on metabolic and bariatric surgery. Obes Surg 2014 Jan;24(1):42-55. [CrossRef] [Medline]
- Heber D, Greenway F, Kaplan L, Livingston E, Salvador J, Still C, et al. Endocrine and nutritional management of the post-bariatric surgery patient: an Endocrine Society Clinical Practice Guideline. J Clin Endocrinol Metab 2010 Nov;95(11):4823-4843. [CrossRef] [Medline]
- Andreu A, Moizé V, Rodríguez L, Flores L, Vidal J. Protein intake, body composition, and protein status following bariatric surgery. Obes Surg 2010 Nov;20(11):1509-1515. [CrossRef] [Medline]
- Waterlow JC. Metabolic adaptation to low intakes of energy and protein. Annu Rev Nutr 1986;6:495-526. [CrossRef] [Medline]
- Carey D, Pliego G, Raymond RL. Body composition and metabolic changes following bariatric surgery: effects on fat mass, lean mass and basal metabolic rate: six months to one-year follow-up. Obes Surg 2006 Dec;16(12):1602-1608. [CrossRef] [Medline]
- Cunningham JJ. Body composition as a determinant of energy expenditure: a synthetic review and a proposed general prediction equation. Am J Clin Nutr 1991 Dec;54(6):963-969. [Medline]
- Astrup A, Gøtzsche PC, van de Werken K, Ranneries C, Toubro S, Raben A, et al. Meta-analysis of resting metabolic rate in formerly obese subjects. Am J Clin Nutr 1999 Jun;69(6):1117-1122 [FREE Full text] [Medline]
- Ravussin E, Lillioja S, Knowler W, Christin L, Freymond D, Abbott W, et al. Reduced rate of energy expenditure as a risk factor for body-weight gain. N Engl J Med 1988 Feb 25;318(8):467-472. [CrossRef] [Medline]
- Swenson B, Saalwachter SA, Edwards M, Gross M, Hedrick T, Weltman A, et al. The effect of a low-carbohydrate, high-protein diet on post laparoscopic gastric bypass weight loss: a prospective randomized trial. J Surg Res 2007 Oct;142(2):308-313. [CrossRef] [Medline]
- Schollenberger A, Karschin J, Meile T, Küper MA, Königsrainer A, Bischoff SC. Impact of protein supplementation after bariatric surgery: a randomized controlled double-blind pilot study. Nutrition 2016 Feb;32(2):186-192. [CrossRef] [Medline]
- Clements RH, Saraf N, Kakade M, Yellumahanthi K, White M, Hackett JA. Nutritional effect of oral supplement enriched in beta-hydroxy-beta-methylbutyrate, glutamine and arginine on resting metabolic rate after laparoscopic gastric bypass. Surg Endosc 2011 May;25(5):1376-1382 [FREE Full text] [CrossRef] [Medline]
- Raftopoulos I, Bernstein B, O'Hara K, Ruby JA, Chhatrala R, Carty J. Protein intake compliance of morbidly obese patients undergoing bariatric surgery and its effect on weight loss and biochemical parameters. Surg Obes Relat Dis 2011;7(6):733-742. [CrossRef] [Medline]
- Nair KS, Halliday D, Garrow JS. Thermic response to isoenergetic protein, carbohydrate or fat meals in lean and obese subjects. Clin Sci (Lond) 1983 Sep;65(3):307-312. [Medline]
- Beckman LM, Beckman TR, Sibley SD, Thomas W, Ikramuddin S, Kellogg TA, et al. Changes in gastrointestinal hormones and leptin after Roux-en-Y gastric bypass surgery. JPEN J Parenter Enteral Nutr 2011 Mar;35(2):169-180 [FREE Full text] [CrossRef] [Medline]
- Pacy PJ, Price GM, Halliday D, Quevedo MR, Millward DJ. Nitrogen homeostasis in man: the diurnal responses of protein synthesis and degradation and amino acid oxidation to diets with increasing protein intakes. Clin Sci (Lond) 1994 Jan;86(1):103-116. [Medline]
- Carbone J, McClung J, Pasiakos SM. Skeletal muscle responses to negative energy balance: effects of dietary protein. Adv Nutr 2012 Mar 01;3(2):119-126 [FREE Full text] [CrossRef] [Medline]
- [No authors listed]. Energy and protein requirements: report of a joint FAO-WHO ad hoc expert committee. Rome, 22 March - 2 April 1971. World Health Organ Tech Rep Ser 1973;522:1-118. [Medline]
- EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA). Scientific opinion on dietary reference values for energy. EFSA Journal 2013 Jan 10;11(1):3005-3206. [CrossRef]
- Bojsen-Møller KN, Jacobsen SH, Dirksen C, Jørgensen NB, Reitelseder S, Jensen JE, et al. Accelerated protein digestion and amino acid absorption after Roux-en-Y gastric bypass. Am J Clin Nutr 2015 Sep;102(3):600-607 [FREE Full text] [CrossRef] [Medline]
- Felig P, Marliss E, Cahill GF. Plasma amino acid levels and insulin secretion in obesity. N Engl J Med 1969 Oct 9;281(15):811-816. [CrossRef] [Medline]
- Laferrère B, Reilly D, Arias S, Swerdlow N, Gorroochurn P, Bawa B, et al. Differential metabolic impact of gastric bypass surgery versus dietary intervention in obese diabetic subjects despite identical weight loss. Sci Transl Med 2011 Apr 27;3(80):80re2 [FREE Full text] [CrossRef] [Medline]
- Tan H, Khoo C, Tan M, Kovalik J, Ng AC, Eng AK, et al. The effects of sleeve gastrectomy and gastric bypass on branched-chain amino acid metabolism 1 year after bariatric surgery. Obes Surg 2016 Aug;26(8):1830-1835. [CrossRef] [Medline]
- Westerterp-Plantenga MS, Lemmens SG, Westerterp KR. Dietary protein - its role in satiety, energetics, weight loss and health. Br J Nutr 2012 Aug;108 Suppl 2:S105-S112. [CrossRef] [Medline]
- Leidy HJ, Carnell NS, Mattes RD, Campbell WW. Higher protein intake preserves lean mass and satiety with weight loss in pre-obese and obese women. Obesity (Silver Spring) 2007 Feb;15(2):421-429 [FREE Full text] [CrossRef] [Medline]
- Martens EA, Westerterp-Plantenga MS. Protein diets, body weight loss and weight maintenance. Curr Opin Clin Nutr Metab Care 2014 Jan;17(1):75-79. [CrossRef] [Medline]
- Yousseif A, Emmanuel J, Karra E, Millet Q, Elkalaawy M, Jenkinson AD, et al. Differential effects of laparoscopic sleeve gastrectomy and laparoscopic gastric bypass on appetite, circulating acyl-ghrelin, peptide YY3-36 and active GLP-1 levels in non-diabetic humans. Obes Surg 2014 Feb;24(2):241-252 [FREE Full text] [CrossRef] [Medline]
- Blom WA, Lluch A, Stafleu A, Vinoy S, Holst J, Schaafsma G, et al. Effect of a high-protein breakfast on the postprandial ghrelin response. Am J Clin Nutr 2006 Feb;83(2):211-220 [FREE Full text] [Medline]
- [No authors listed]. Dietary protein quality evaluation in human nutrition. Report of an FAQ expert consultation. FAO Food Nutr Pap 2013;92:1-66. [Medline]
- Pennings B, Boirie Y, Senden JM, Gijsen AP, Kuipers H, van Loon LJ. Whey protein stimulates postprandial muscle protein accretion more effectively than do casein and casein hydrolysate in older men. Am J Clin Nutr 2011 May;93(5):997-1005 [FREE Full text] [CrossRef] [Medline]
- Walrand S, Gryson C, Salles J, Giraudet C, Migné C, Bonhomme C, et al. Fast-digestive protein supplement for ten days overcomes muscle anabolic resistance in healthy elderly men. Clin Nutr 2016 Jun;35(3):660-668. [CrossRef] [Medline]
- Coles L, editor. Clinical Nutrition: The Interface Between Metabolism, Diet, and Disease. Ontario, Canada: Apple Academic Press, Inc; 2014.
- A Report of the Panel on Macronutrients, Subcommittees on Upper Reference Levels of Nutrients and Interpretation and Uses of Dietary Reference Intakes, Standing Committee on the Scientific Evaluation of Dietary Reference Intakes, Food and Nutrition Board, Institute of Medicine. Dietary reference intakes for energy, carbohydrate, fiber, fat, fatty acids, cholesterol, protein, and amino acids. Washington, DC: National Academies Press; Nov 28, 2005.
- University of Minnesota. Nutrition Data System for Research Nutritional Analysis Software. Nutritional Analysis Software. 2011. URL: http://license.umn.edu/technologies/ndsr87072_nutrition-data-system-for-research-nutritional-analysis-software [accessed 2016-10-27] [WebCite Cache]
- Hoffer LJ, Bistrian BR, Young VR, Blackburn GL, Matthews DE. Metabolic effects of very low calorie weight reduction diets. J Clin Invest 1984 Mar;73(3):750-758 [FREE Full text] [CrossRef] [Medline]
- Wilkinson R. Polyethylene glycol 4000 as a continuously administered non-absorbable faecal marker for metabolic balance studies in human subjects. Gut 1971 Aug;12(8):654-660 [FREE Full text] [Medline]
- Allen LH, Raynolds WL, Margen S. Polyethylene glycol as a quantitative fecal marker in human nutrition experiments. Am J Clin Nutr 1979 Feb;32(2):427-440. [Medline]
- Levitt DG, Beckman LM, Mager JR, Valentine B, Sibley SD, Beckman TR, et al. Comparison of DXA and water measurements of body fat following gastric bypass surgery and a physiological model of body water, fat, and muscle composition. J Appl Physiol (1985) 2010 Sep;109(3):786-795 [FREE Full text] [CrossRef] [Medline]
- Widen EM, Strain G, King WC, Yu W, Lin S, Goodpaster B, et al. Validity of bioelectrical impedance analysis for measuring changes in body water and percent fat after bariatric surgery. Obes Surg 2014 Jun;24(6):847-854 [FREE Full text] [CrossRef] [Medline]
- Strain GW, Gagner M, Pomp A, Dakin G, Inabnet WB, Saif T. Comparison of fat-free mass in super obesity (BMI ≥ 50 kg/m2) and morbid obesity (BMI <50 kg/m2) in response to different weight loss surgeries. Surg Obes Relat Dis 2012;8(3):255-259. [CrossRef] [Medline]
- Mazariegos M, Kral JG, Wang J, Waki M, Heymsfield SB, Pierson RN, et al. Body composition and surgical treatment of obesity. Effects of weight loss on fluid distribution. Ann Surg 1992 Jul;216(1):69-73. [Medline]
- Silva AM, Shen W, Wang Z, Aloia JF, Nelson ME, Heymsfield SB, et al. Three-compartment model: critical evaluation based on neutron activation analysis. Am J Physiol Endocrinol Metab 2004 Nov;287(5):E962-E969 [FREE Full text] [CrossRef] [Medline]
- Das SK, Roberts SB, McCrory MA, Hsu LK, Shikora SA, Kehayias JJ, et al. Long-term changes in energy expenditure and body composition after massive weight loss induced by gastric bypass surgery. Am J Clin Nutr 2003 Jul;78(1):22-30 [FREE Full text] [Medline]
- Rosenbaum M, Ravussin E, Matthews D, Gilker C, Ferraro R, Heymsfield S, et al. A comparative study of different means of assessing long-term energy expenditure in humans. Am J Physiol 1996 Mar;270(3 Pt 2):R496-R504. [Medline]
- Flint A, Raben A, Blundell JE, Astrup A. Reproducibility, power and validity of visual analogue scales in assessment of appetite sensations in single test meal studies. Int J Obes Relat Metab Disord 2000 Jan;24(1):38-48. [Medline]
- Epel E, Tomiyama A, Mason A, Laraia B, Hartman W, Ready K, et al. The reward-based eating drive scale: a self-report index of reward-based eating. PLoS One 2014;9(6):e101350 [FREE Full text] [CrossRef] [Medline]
- Newgard C, An J, Bain J, Muehlbauer M, Stevens R, Lien L, et al. A branched-chain amino acid-related metabolic signature that differentiates obese and lean humans and contributes to insulin resistance. Cell Metab 2009 Apr;9(4):311-326 [FREE Full text] [CrossRef] [Medline]
- DeFronzo RA, Matsuda M. Reduced time points to calculate the composite index. Diabetes Care 2010 Jul;33(7):e93. [CrossRef] [Medline]
- Baba NH, Sawaya S, Torbay N, Habbal Z, Azar S, Hashim SA. High protein vs high carbohydrate hypoenergetic diet for the treatment of obese hyperinsulinemic subjects. Int J Obes Relat Metab Disord 1999 Nov;23(11):1202-1206. [Medline]
- Koltz PF, Chen R, Messing S, Gusenoff JA. Prospective assessment of nutrition and exercise parameters before body contouring surgery: optimizing attainability in the massive weight loss population. Plast Reconstr Surg 2010 Apr;125(4):1242-1247. [CrossRef] [Medline]
- Lips MA, Van Klinken JB, van Harmelen V, Dharuri HK, 't Hoen PA, Laros JF, et al. Roux-en-Y gastric bypass surgery, but not calorie restriction, reduces plasma branched-chain amino acids in obese women independent of weight loss or the presence of type 2 diabetes. Diabetes Care 2014 Dec;37(12):3150-3156. [CrossRef] [Medline]
- She P, Van HC, Reid T, Hutson SM, Cooney RN, Lynch CJ. Obesity-related elevations in plasma leucine are associated with alterations in enzymes involved in branched-chain amino acid metabolism. Am J Physiol Endocrinol Metab 2007 Dec;293(6):E1552-E1563 [FREE Full text] [CrossRef] [Medline]
- Tremblay F, Jacques H, Marette A. Modulation of insulin action by dietary proteins and amino acids: role of the mammalian target of rapamycin nutrient sensing pathway. Curr Opin Clin Nutr Metab Care 2005 Jul;8(4):457-462. [Medline]
- Wang TJ, Larson MG, Vasan RS, Cheng S, Rhee EP, McCabe E, et al. Metabolite profiles and the risk of developing diabetes. Nat Med 2011 Apr;17(4):448-453 [FREE Full text] [CrossRef] [Medline]
- Shimomura Y, Yamamoto Y, Bajotto G, Sato J, Murakami T, Shimomura N, et al. Nutraceutical effects of branched-chain amino acids on skeletal muscle. J Nutr 2006 Feb;136(2):529S-532S [FREE Full text] [Medline]
- Pal S, Ellis V, Dhaliwal S. Effects of whey protein isolate on body composition, lipids, insulin and glucose in overweight and obese individuals. Br J Nutr 2010 Sep;104(5):716-723. [CrossRef] [Medline]
|AA: amino acid|
|BC: body composition|
|BCAA: branched chain amino acid|
|BS: bariatric surgery|
|CRR: Clinical Research Resource|
|D2O: deuterium oxide|
|DIT: diet-induced thermogenesis|
|EE: energy expenditure|
|FFM: fat-free mass|
|FM: fat mass|
|GBP: gastric bypass|
|GLP-1: glucagon-like peptide 1|
|HPS-G: high protein supplementation group|
|IBW: ideal body weight|
|IR: insulin resistance|
|LBM: lean body mass|
|NB: nitrogen balance|
|NDSR: Nutrition Data System for Research|
|PEG: polyethylene glycol|
|PI: protein intake|
|PS: protein supplementation|
|PYY: peptide YY|
|RCT: randomized controlled trial|
|REE: resting energy expenditure|
|SD: standard deviation|
|SG: sleeve gastrectomy|
|SPS-G: standard protein supplementation group|
|T2DM: type 2 diabetes mellitus|
|TBW: total body water|
|VAS: visual analog scale|
Edited by G Eysenbach; submitted 03.08.16; peer-reviewed by MS Westerterp-Plantenga, Y Liu; comments to author 31.08.16; revised version received 16.09.16; accepted 17.09.16; published 28.11.16
©Violeta Moizé, Xavier Pi-Sunyer, Josep Vidal, Patricia Miner, Yves Boirie, Blandine Laferrère. Originally published in JMIR Research Protocols (http://www.researchprotocols.org), 28.11.2016.
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