%0 Journal Article
%@ 1929-0748
%I JMIR Publications Inc.
%V 4
%N 3
%P e89
%T Interleukin 2 Topical Cream for Treatment of Diabetic Foot Ulcer: Experiment Protocol
%A Chan,Shu Wing Sophia
%+ currently no institutional affiliation, sophiachan325@hotmail.com
%K type 1 diabetes
%K topical cream
%K chronic wound healing
%K immunotherapy
%K IL-2
%D 2015
%7 14.08.2015
%9 Protocol
%J JMIR Res Protoc
%G English
%X Background: It is estimated there are 2.9 million diabetic patients in the United Kingdom, and around 5%-7% of patients have diabetic ulcers. This number will continue to increase globally. Diabetic ulcers are a major economic burden on the healthcare system. More than £650 million is spent on foot ulcers or amputations each year, and up to 100 people a week have a limb amputated due to diabetes.  In T1DM, the level of IL-2 is reduced, and hence, wound healing is in a prolonged inflammatory phase. It is not known if IL-2 topical cream can shorten the healing process in T1DM patients. Objective: The objective of this study is to understand the pathophysiology in type 1 diabetes (T1DM) and investigate possible future treatment based on its clinical features. The hypothesis is that IL-2 cream can speed up wound healing in NOD mice and that this can be demonstrated in a ten-week study. An experiment protocol is designed in a mouse model for others to conduct the experiment. The discussion is purely based on diabetic conditions; lifestyle influences like smoking and drinking are not considered. Methods: Skin incisions will be created on 20 nonobese diabetic (NOD) mice, and IL-2 topical cream will be applied in a 10-week study to prove the hypothesis. Mice will be randomly and equally divide into two groups with one being the control group. Results: T1DM patients have a decreased number of T regulatory (Treg) cells and interleukin 2 (IL-2). These are the keys to the disease progression and delay in wound healing. Diabetic ulcer is a chronic wound and characterized by a prolonged inflammatory phase. Conclusions: If the experiment is successful, T1DM patients will have an alternative, noninvasive treatment of foot ulcers. In theory, patients with other autoimmune diseases could also use IL-2 topical cream for treatment. 
%M 26276522
%R 10.2196/resprot.4036
%U http://www.researchprotocols.org/2015/3/e89/
%U https://doi.org/10.2196/resprot.4036
%U http://www.ncbi.nlm.nih.gov/pubmed/26276522