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  <front>
    <journal-meta>
      <journal-id journal-id-type="publisher-id">ResProt</journal-id>
      <journal-id journal-id-type="nlm-ta">JMIR Res Protoc</journal-id>
      <journal-title>JMIR Research Protocols</journal-title>
      <issn pub-type="epub">1929-0748</issn>
      <publisher>
        <publisher-name>JMIR Publications</publisher-name>
        <publisher-loc>Toronto, Canada</publisher-loc>
      </publisher>
    </journal-meta>
    <article-meta>
      <article-id pub-id-type="publisher-id">v13i1e54254</article-id>
      <article-id pub-id-type="pmid">38652533</article-id>
      <article-id pub-id-type="doi">10.2196/54254</article-id>
      <article-categories>
        <subj-group subj-group-type="heading">
          <subject>Protocol</subject>
        </subj-group>
        <subj-group subj-group-type="article-type">
          <subject>Protocol</subject>
        </subj-group>
      </article-categories>
      <title-group>
        <article-title>Testing the Effectiveness of an Intervention to Improve Romanian Teachers’ LGBT+-Related Attitudes, Cognitions, Behaviors, and Affect: Protocol for a Randomized Controlled Trial</article-title>
      </title-group>
      <contrib-group>
        <contrib contrib-type="editor">
          <name>
            <surname>Mavragani</surname>
            <given-names>Amaryllis</given-names>
          </name>
        </contrib>
      </contrib-group>
      <contrib-group>
        <contrib contrib-type="reviewer">
          <name>
            <surname>Rendina</surname>
            <given-names>Jonathon</given-names>
          </name>
        </contrib>
        <contrib contrib-type="reviewer">
          <name>
            <surname>Xu</surname>
            <given-names>Jackie</given-names>
          </name>
        </contrib>
      </contrib-group>
      <contrib-group>
        <contrib id="contrib1" contrib-type="author" corresp="yes">
          <name name-style="western">
            <surname>Latu</surname>
            <given-names>Ioana</given-names>
          </name>
          <degrees>PhD</degrees>
          <xref rid="aff1" ref-type="aff">1</xref>
          <address>
            <institution>Department of Psychology</institution>
            <institution>Faculty of Sociology and Psychology</institution>
            <institution>West University of Timisoara</institution>
            <addr-line>Bulevardul Vasile Pârvan 4</addr-line>
            <addr-line>Timisoara, 300223</addr-line>
            <country>Romania</country>
            <phone>40 7901037222</phone>
            <email>i.latu@qub.ac.uk</email>
          </address>
          <xref rid="aff2" ref-type="aff">2</xref>
          <ext-link ext-link-type="orcid">https://orcid.org/0000-0003-3328-9261</ext-link>
        </contrib>
        <contrib id="contrib2" contrib-type="author">
          <name name-style="western">
            <surname>Sălăgean</surname>
            <given-names>Nastasia</given-names>
          </name>
          <degrees>PhD</degrees>
          <xref rid="aff3" ref-type="aff">3</xref>
          <ext-link ext-link-type="orcid">https://orcid.org/0000-0002-9923-1559</ext-link>
        </contrib>
        <contrib id="contrib3" contrib-type="author">
          <name name-style="western">
            <surname>Larsen</surname>
            <given-names>Torill M B</given-names>
          </name>
          <degrees>PhD</degrees>
          <xref rid="aff4" ref-type="aff">4</xref>
          <ext-link ext-link-type="orcid">https://orcid.org/0000-0003-3457-9922</ext-link>
        </contrib>
        <contrib id="contrib4" contrib-type="author">
          <name name-style="western">
            <surname>Isbasoiu</surname>
            <given-names>Andreea Bogdana</given-names>
          </name>
          <degrees>PhD</degrees>
          <xref rid="aff5" ref-type="aff">5</xref>
          <ext-link ext-link-type="orcid">https://orcid.org/0000-0003-0377-9358</ext-link>
        </contrib>
        <contrib id="contrib5" contrib-type="author">
          <name name-style="western">
            <surname>Sava</surname>
            <given-names>Florin Alin</given-names>
          </name>
          <degrees>PhD</degrees>
          <xref rid="aff1" ref-type="aff">1</xref>
          <ext-link ext-link-type="orcid">https://orcid.org/0000-0001-8898-1306</ext-link>
        </contrib>
      </contrib-group>
      <aff id="aff1">
        <label>1</label>
        <institution>Department of Psychology</institution>
        <institution>Faculty of Sociology and Psychology</institution>
        <institution>West University of Timisoara</institution>
        <addr-line>Timisoara</addr-line>
        <country>Romania</country>
      </aff>
      <aff id="aff2">
        <label>2</label>
        <institution>School of Psychology</institution>
        <institution>Queen's University Beflast</institution>
        <addr-line>Beflast</addr-line>
        <country>United Kingdom</country>
      </aff>
      <aff id="aff3">
        <label>3</label>
        <institution>Department of Scientific Research in Economy, Law and Human-Environment Interaction</institution>
        <institution>Institute for Advanced Environmental Research</institution>
        <institution>West University of Timișoara</institution>
        <addr-line>Timisoara</addr-line>
        <country>Romania</country>
      </aff>
      <aff id="aff4">
        <label>4</label>
        <institution>Department of Health Promotion and Development</institution>
        <institution>Faculty of Psychology at University of Bergen</institution>
        <addr-line>Bergen</addr-line>
        <country>Norway</country>
      </aff>
      <aff id="aff5">
        <label>5</label>
        <institution>Department of Psychology and Education Sciences</institution>
        <institution>Transilvania University of Brasov</institution>
        <addr-line>Brasov</addr-line>
        <country>Romania</country>
      </aff>
      <author-notes>
        <corresp>Corresponding Author: Ioana Latu <email>i.latu@qub.ac.uk</email></corresp>
      </author-notes>
      <pub-date pub-type="collection">
        <year>2024</year>
      </pub-date>
      <pub-date pub-type="epub">
        <day>23</day>
        <month>4</month>
        <year>2024</year>
      </pub-date>
      <volume>13</volume>
      <elocation-id>e54254</elocation-id>
      <history>
        <date date-type="received">
          <day>2</day>
          <month>11</month>
          <year>2023</year>
        </date>
        <date date-type="rev-request">
          <day>5</day>
          <month>2</month>
          <year>2024</year>
        </date>
        <date date-type="rev-recd">
          <day>7</day>
          <month>2</month>
          <year>2024</year>
        </date>
        <date date-type="accepted">
          <day>4</day>
          <month>3</month>
          <year>2024</year>
        </date>
      </history>
      <copyright-statement>©Ioana Latu, Nastasia Sălăgean, Torill M B Larsen, Andreea Bogdana Isbasoiu, Florin Alin Sava. Originally published in JMIR Research Protocols (https://www.researchprotocols.org), 23.04.2024.</copyright-statement>
      <copyright-year>2024</copyright-year>
      <license license-type="open-access" xlink:href="https://creativecommons.org/licenses/by/4.0/">
        <p>This is an open-access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work, first published in JMIR Research Protocols, is properly cited. The complete bibliographic information, a link to the original publication on https://www.researchprotocols.org, as well as this copyright and license information must be included.</p>
      </license>
      <self-uri xlink:href="https://www.researchprotocols.org/2024/1/e54254" xlink:type="simple"/>
      <abstract>
        <sec sec-type="background">
          <title>Background</title>
          <p>Repeated stigmatization due to group membership constitutes a recurrent stressor with negative impact on physical and mental health (minority stress model). Among European countries, Romania ranks low on LGBT+ (lesbian, gay, bisexual, and transgender people. The “+” represents individuals whose identities do not fit typical binary notions of male and female [nonbinary]) inclusion, with 45% of Romanian LGBT+ respondents reporting discrimination in at least 1 area of life in the year preceding the survey. Importantly, while all LGBT+ people might experience minority stress, younger sexual minority individuals are more prone to the detrimental impacts of stigma on their mental and physical health. As such, interventions are necessary to improve the inclusion climate within schools, where young people spend most of their time. Until now, most interventions addressing this topic have been conducted on undergraduate students in Western countries, with no studies conducted in countries that have widespread anti-LGBT+ attitudes.</p>
        </sec>
        <sec sec-type="objective">
          <title>Objective</title>
          <p>This paper describes the research protocol for a randomized controlled trial investigating whether LGBT+ stigma and bias among Romanian school teachers can be reduced using an internet-based intervention focusing on education and contact as primary training elements.</p>
        </sec>
        <sec sec-type="methods">
          <title>Methods</title>
          <p>A sample of 175 school teachers will be randomly assigned to either the control or experimental group. The experimental group participants will receive the intervention first and then complete the outcome measures, whereas the control group will complete the outcome measures first and then receive the intervention. The 1-hour multimedia intervention is developed for internet-based delivery under controlled conditions. It includes 2 interactive exercises, 2 recorded presentations, animations, and testimonies from LGBT+ individuals. Data for attitudinal, behavioral, cognitive, and affective measures will be collected during the same session (before or after the intervention, depending on the condition). We also plan to conduct a brief mixed methods follow-up study at 6 to 8 months post participation to investigate potential long-term effects of training. However, due to attrition and lack of experimental control (all participants will have completed the intervention, regardless of the condition), these data will be analyzed and reported separately using a mixed methods approach.</p>
        </sec>
        <sec sec-type="results">
          <title>Results</title>
          <p>This paper details the protocol for the teacher intervention study. Data collection began in December 2022 and was completed by February 2023. Data analysis will be performed upon protocol acceptance. Follow-up measures will be completed in 2024. Results are expected to be submitted for publication following analysis in the spring of 2024.</p>
        </sec>
        <sec sec-type="conclusions">
          <title>Conclusions</title>
          <p>The findings of this study will establish the effectiveness of an internet-based intervention intended to lessen anti-LGBT stigma and sentiment in a nation where these views have long been prevalent. If successful, the intervention could end up serving as a resource for Romanian teachers and guidance counselors in high schools.</p>
        </sec>
        <sec sec-type="Trial Registration">
          <title>Trial Registration</title>
          <p>ISRCTN 84290049; https://doi.org/10.1186/ISRCTN84290049</p>
        </sec>
        <sec sec-type="registered-report">
          <title>International Registered Report Identifier (IRRID)</title>
          <p>DERR1-10.2196/54254</p>
        </sec>
      </abstract>
      <kwd-group>
        <kwd>discrimination</kwd>
        <kwd>intervention</kwd>
        <kwd>school</kwd>
        <kwd>lesbian, gay, bisexual, and transgender</kwd>
        <kwd>attitude</kwd>
        <kwd>behavior</kwd>
        <kwd>cognition</kwd>
        <kwd>stigma</kwd>
        <kwd>stigmatization</kwd>
        <kwd>negative impact</kwd>
        <kwd>physical health</kwd>
        <kwd>mental health</kwd>
        <kwd>minority stress model</kwd>
        <kwd>European</kwd>
        <kwd>Europe</kwd>
        <kwd>Romania</kwd>
        <kwd>stress</kwd>
        <kwd>young</kwd>
        <kwd>student</kwd>
        <kwd>students</kwd>
        <kwd>undergraduate</kwd>
        <kwd>bias</kwd>
        <kwd>data analysis</kwd>
        <kwd>online intervention</kwd>
        <kwd>lesbian, gay, bisexual, and transgender stigma</kwd>
        <kwd>sentiment</kwd>
      </kwd-group>
    </article-meta>
  </front>
  <body>
    <sec sec-type="introduction">
      <title>Introduction</title>
      <sec>
        <title>Overview</title>
        <p>The experience of stigmatization based on group membership can lead to negative effects on physical and mental health via repeated experiences of stress which, in the long term, accumulate to impact health negatively (minority stress model) [<xref ref-type="bibr" rid="ref1">1</xref>]. Stigmatization can occur based on membership in any group that is discriminated against. In this research, we focus on LGBT+ (lesbian, gay, bisexual, and transgender people. The “+” symbol represents individuals whose identities do not fit typical binary notions of male and female [nonbinary]) stigmatization and its reduction in Romania.</p>
        <p>Among European countries, Romania tends to fare poorly when it comes to LGBT+ inclusion. A recent survey [<xref ref-type="bibr" rid="ref2">2</xref>] showed that 45% of Romanian LGBT+ respondents felt discriminated against in at least 1 area of life during the year before the survey. Similarly, 43% of respondents experienced harassment due to being LGBT+, a rate that ranks second among all European countries. Much of this discrimination and harassment happens in schools for young people, with 44% of LGBT+ students (15-17 years of age) in Romania saying they are hiding being LGBT+ at school, a rate that is 3 to 4 times higher than in countries such as Luxembourg (11%), the Netherlands (16%), or Malta (17%). Indeed, schools seem to be a fertile ground for LGBT+ harassment, with 51% of incidents involving perpetrators from schools [<xref ref-type="bibr" rid="ref2">2</xref>], a finding reinforced by a UNESCO (The United Nations Educational, Scientific and Cultural Organization) report on school violence [<xref ref-type="bibr" rid="ref3">3</xref>].</p>
        <p>Such high rates of LGBT+ discrimination among Romanian students are concerning, because stigma impacts young LGBT+ people’s mental and physical health. A meta-analysis [<xref ref-type="bibr" rid="ref4">4</xref>] including 35 studies of over 2 million heterosexual young people and over 100,000 LGBT+ young individuals (age range 12-20 years) showed that suicide risk was higher for LGBT+ young people compared to those who were not LGBT+. For every heterosexual young person, the chance of suicide was 3.71 higher for gay and lesbian young people, 4.87 higher for bisexual youth, and 5.87 higher for young transgender people. This risk is paired with a higher likelihood for sexual minority youth to experience mental health issues such as anxiety and depression but also impaired academic performance [<xref ref-type="bibr" rid="ref5">5</xref>]. The findings for sexual minority youth are consistent with sexual minority individuals in general—a systematic review showed that the majority of studies revealed a higher risk for mental health issues, substance abuse, and suicide risk for sexual minorities [<xref ref-type="bibr" rid="ref6">6</xref>]. LGBT+ people are also more likely to endure physical health issues due to minority stress, including an increased risk for cancer, cardiovascular disease, and chronic conditions such as asthma and diabetes [<xref ref-type="bibr" rid="ref7">7</xref>].</p>
        <p>Importantly, although minority stress can affect all LGBT+ people, younger sexual minority individuals seem to be most vulnerable to the negative effects of stigma on their mental and physical health. A meta-analysis indicated age as a significant moderator, such that youth younger than 17 years of age experienced higher negative outcomes due to LGBT-related victimization compared to those older than 17 years of age [<xref ref-type="bibr" rid="ref8">8</xref>].</p>
        <p>Together, these findings indicate a strong need to implement effective interventions to reduce LGBT+ stigma and bias in Romanian schools, to improve the mental and physical health of LGBT+ students in Romania. These interventions may be most efficient particularly among teachers, as they can impact their students’ experiences either directly or indirectly, via establishing inclusivity norms in the classroom.</p>
        <p>To be effective, interventions should not only focus on reducing teachers’ biases toward LGBT+ students, but also on learning, implementing, and modeling behaviors that would equip them to intervene when students experience LGBT+-related victimization. A meta-analysis [<xref ref-type="bibr" rid="ref9">9</xref>] showed that interventions can be moderately successful in reducing sexual prejudice, resulting in changes ranging from one-third to one-half of an SD in size. Moreover, the meta-analysis specified the most effective strategies in reducing different outcomes—educational interventions, contact with LGBT+ individuals, and interventions combining education with contact. A more careful analysis reveals that most intervention studies have been conducted on undergraduate students in Western countries and none in Eastern Europe or other countries with strong antigay attitudes. Although more recent research has started to test interventions in other countries such as Jamaica [<xref ref-type="bibr" rid="ref10">10</xref>] or Brazil [<xref ref-type="bibr" rid="ref11">11</xref>], no such adaptation exists in Romania (or Eastern Europe), particularly for teachers. Adapting interventions to the cultural and institutional context seems vital, given that a recent qualitative analysis suggests that participants tend to criticize many interventions for their mismatch with the context in which they are conducted, possibly as a rationale for resisting change [<xref ref-type="bibr" rid="ref12">12</xref>].</p>
        <p>The primary goal of this research line is to design and test an intervention that is geared toward Romanian teachers. Importantly, we plan to achieve this goal by deliberately taking into account specific cultural and institutional characteristics, rather than indiscriminately applying previous intervention strategies. More specifically, although we plan to use education and contact as primary training elements, consistent with meta-analytic findings [<xref ref-type="bibr" rid="ref9">9</xref>], we plan to design the educational components to respond to the needs of our particular target group. For example, we drew from recent research conducted in Russia [<xref ref-type="bibr" rid="ref13">13</xref>], a country with a similar culture and history regarding LGBT+ attitudes. Building on these findings, we propose that the educational component should include information regarding the biological (vs social) causes of homosexuality, given that attributions of causality are related to perceived threat, and subsequently lead to biased outcomes. In addition, given the importance of threat in predicting antigay bias, and consistent with intergroup threat theory [<xref ref-type="bibr" rid="ref14">14</xref>], education should explicitly address potential feelings or perceptions of threat (for example, the perception that exposure to LGBT+ people will “make” children gay).</p>
        <p>A second novel aspect is that the intervention will aim not only to improve attitudes and knowledge as an outcome but also target behavioral change by imparting tools that teachers can use to address LGBT+-related victimization in schools. This strategy has been used successfully in the past in a web-based intervention program for Brazilian health practitioners [<xref ref-type="bibr" rid="ref11">11</xref>]. As a result, we are likely to increase the impact of the intervention of LGBT+ students, not only by improving the inclusion climate within the schools but also by improving teachers’ actual behavioral intentions and skills that may improve LGBT+ students’ outcomes.</p>
      </sec>
      <sec>
        <title>Aims and Hypotheses</title>
        <p>In this research, we design and report the protocol of a randomized controlled trial (RCT) intervention and evaluation. The intervention is based on existing evidence about the efficacy of strategies identified in Bartoş et al’s [<xref ref-type="bibr" rid="ref9">9</xref>] meta-analysis. According to their results, the most effective strategies in reducing LGBT+ stigma combine educational elements with contact with LGBT+ people. We also add elements that were found to be useful in other bias-reducing interventions such as perspective taking [<xref ref-type="bibr" rid="ref15">15</xref>] and self-efficacy [<xref ref-type="bibr" rid="ref16">16</xref>,<xref ref-type="bibr" rid="ref17">17</xref>], and which we deem culturally appropriate. A summary of intervention components, subcomponents, and contents is presented in <xref ref-type="table" rid="table1">Table 1</xref>. For each element, we include the supporting reference, as an evidence base for their efficiency in reducing bias.</p>
        <p>Using an RCT in which teachers are randomly assigned to complete the intervention before assessing outcomes (experimental) or after assessing outcomes (control), we predict that the intervention will have a positive effect on attitudinal, cognitive, behavioral, and affective measures of LGBT+ bias among teachers. Specifically, we predict that teachers randomly assigned to the experimental condition, compared to those randomly assigned to the control condition will experience (1) more positive attitudes toward LGBT+ individuals, (2) more factual knowledge about LGBT+ issues, (3) stronger behavioral intentions and self-efficacy about addressing LGBT+ issues in the classroom, and (4) more positive affect toward LGBT+ individuals.</p>
        <table-wrap position="float" id="table1">
          <label>Table 1</label>
          <caption>
            <p>Training elements for the randomized controlled trial intervention designed to improve Romanian teachers’ LGBT+<sup>a</sup> related attitudes, cognitions, behaviors, and affect<sup>b</sup>.</p>
          </caption>
          <table width="1000" cellpadding="5" cellspacing="0" border="1" rules="groups" frame="hsides">
            <col width="30"/>
            <col width="200"/>
            <col width="290"/>
            <col width="480"/>
            <thead>
              <tr valign="top">
                <td colspan="2">Intervention component and subcomponent</td>
                <td>Duration and timestamps</td>
                <td>Content and supporting references</td>
              </tr>
            </thead>
            <tbody>
              <tr valign="top">
                <td colspan="2">Introduction</td>
                <td>
                  <list list-type="bullet">
                    <list-item>
                      <p>Duration: 02 minutes 50 seconds</p>
                    </list-item>
                    <list-item>
                      <p>Timestamps: 00.00-02.50</p>
                    </list-item>
                  </list>
                </td>
                <td>Introduction from academic research team, highlighting the goals and the evidence-based nature of the intervention</td>
              </tr>
              <tr valign="top">
                <td colspan="4">
                  <bold>Education</bold>
                  <bold>[<xref ref-type="bibr" rid="ref9">9</xref>]</bold>
                </td>
              </tr>
              <tr valign="top">
                <td>
                  <break/>
                </td>
                <td>Definition of terms</td>
                <td>
                  <list list-type="bullet">
                    <list-item>
                      <p>Duration: 05 minutes 40 seconds</p>
                    </list-item>
                    <list-item>
                      <p>Timestamps: 02.50-08.30</p>
                    </list-item>
                  </list>
                </td>
                <td>
                  <list list-type="bullet">
                    <list-item>
                      <p>Definition of LGBT+ terms</p>
                    </list-item>
                    <list-item>
                      <p>Definition of sexual orientation</p>
                    </list-item>
                    <list-item>
                      <p>Differentiation between sex versus gender</p>
                    </list-item>
                    <list-item>
                      <p>Definition of intersex</p>
                    </list-item>
                    <list-item>
                      <p>Description of gender identity and transgender</p>
                    </list-item>
                  </list>
                </td>
              </tr>
              <tr valign="top">
                <td>
                  <break/>
                </td>
                <td>Threat reduction</td>
                <td>
                  <list list-type="bullet">
                    <list-item>
                      <p>Duration: 03 minutes 40 seconds</p>
                    </list-item>
                    <list-item>
                      <p>Timestamps: 08.30-12.10</p>
                    </list-item>
                  </list>
                </td>
                <td>
                  <list list-type="bullet">
                    <list-item>
                      <p>LGBT+ children and their families: meta-analysis on children raised by LGBT+ families</p>
                    </list-item>
                    <list-item>
                      <p>Sexuality and sexual orientation in humans and animals.</p>
                    </list-item>
                    <list-item>
                      <p>Normalizing varied sexual orientations across species</p>
                    </list-item>
                    <list-item>
                      <p>The function of same-sex sexual behavior in ensuring survival</p>
                    </list-item>
                  </list>
                </td>
              </tr>
              <tr valign="top">
                <td>
                  <break/>
                </td>
                <td>Effects of stigma to understand minority stress [<xref ref-type="bibr" rid="ref1">1</xref>]</td>
                <td>
                  <list list-type="bullet">
                    <list-item>
                      <p>Duration: 05 minutes 35 seconds</p>
                    </list-item>
                    <list-item>
                      <p>Timestamps: 12.10-17.45</p>
                    </list-item>
                  </list>
                </td>
                <td>
                  <list list-type="bullet">
                    <list-item>
                      <p>Suicide rates of LGBT+ youth</p>
                    </list-item>
                    <list-item>
                      <p>The role of stress from exposure to prejudice, ridicule, physical, and verbal aggression in understanding mental health outcomes</p>
                    </list-item>
                    <list-item>
                      <p>The role of social support and family or school acceptance in improving mental health and the danger of conversion therapy</p>
                    </list-item>
                    <list-item>
                      <p>Bullying as a special case of discrimination, types of bullying, the role of sexual orientation</p>
                    </list-item>
                  </list>
                </td>
              </tr>
              <tr valign="top">
                <td>
                  <break/>
                </td>
                <td>Behavioral tools [<xref ref-type="bibr" rid="ref11">11</xref>]</td>
                <td>
                  <list list-type="bullet">
                    <list-item>
                      <p>Duration: 04 minutes 00 second</p>
                    </list-item>
                    <list-item>
                      <p>Timestamps: 24.45-28.45</p>
                    </list-item>
                  </list>
                </td>
                <td>
                  <list list-type="bullet">
                    <list-item>
                      <p>Presentation of tools that can be used by teachers, including the following:</p>
                    </list-item>
                  </list>
                  <list list-type="bullet">
                    <list-item>
                      <p>Romania’s new antibullying law and bullying protocol in schools</p>
                    </list-item>
                    <list-item>
                      <p>Lesson plans to discuss and combat bullying in the classroom</p>
                    </list-item>
                    <list-item>
                      <p>UNICEF<sup>c</sup> intervention model for bullying</p>
                    </list-item>
                    <list-item>
                      <p>Procedure to report bullying and harassment with the Romanian National Council for Combating Discrimination</p>
                    </list-item>
                    <list-item>
                      <p>Support groups for LGBT+ students in Romania</p>
                    </list-item>
                  </list>
                  <list list-type="bullet">
                    <list-item>
                      <p>For all resources links were shared in the presentation and then sent in PDF format to all participants</p>
                    </list-item>
                  </list>
                </td>
              </tr>
              <tr valign="top">
                <td colspan="4">
                  <bold>Perspective taking [<xref ref-type="bibr" rid="ref15">15</xref>]</bold>
                </td>
              </tr>
              <tr valign="top">
                <td>
                  <break/>
                </td>
                <td>Writing exercise, 7 minutes</td>
                <td>
                  <list list-type="bullet">
                    <list-item>
                      <p>Duration: 07 minutes 00 second</p>
                    </list-item>
                    <list-item>
                      <p>Timestamps: 17.45-24.45</p>
                    </list-item>
                  </list>
                </td>
                <td>
                  <list list-type="bullet">
                    <list-item>
                      <p>Writing prompt: “Imagine a day in the life of a gay student—write a few paragraphs about what this student is living and feeling on a school day. What are his/her thoughts and feelings that day? Please write down your answers.”</p>
                    </list-item>
                  </list>
                </td>
              </tr>
              <tr valign="top">
                <td colspan="4">
                  <bold>Contact [<xref ref-type="bibr" rid="ref9">9</xref>,<xref ref-type="bibr" rid="ref10">10</xref>]</bold>
                </td>
              </tr>
              <tr valign="top">
                <td>
                  <break/>
                </td>
                <td>Indirect contact via recorded testimonials</td>
                <td>
                  <list list-type="bullet">
                    <list-item>
                      <p>Duration: 15 minutes 00 second</p>
                    </list-item>
                    <list-item>
                      <p>Timestamps: 28.45-43:45</p>
                    </list-item>
                  </list>
                </td>
                <td>
                  <list list-type="bullet">
                    <list-item>
                      <p>Video testimonials of 3 university students (1 gay man, 1 bisexual woman, and a lesbian woman) and 1 teacher (lesbian woman).</p>
                    </list-item>
                    <list-item>
                      <p>Testimonial prompts for students included the following:</p>
                    </list-item>
                  </list>
                  <list list-type="bullet">
                    <list-item>
                      <p>Tell us about yourself</p>
                    </list-item>
                    <list-item>
                      <p>When did you come out? What were your barriers during this process</p>
                    </list-item>
                    <list-item>
                      <p>How was your experience in school? Were there teachers who supported you?</p>
                    </list-item>
                    <list-item>
                      <p>What would you have needed when you were a high school student?</p>
                    </list-item>
                    <list-item>
                      <p>What would you say to teachers to help them support their LGBT+ students?</p>
                    </list-item>
                  </list>
                  <list list-type="bullet">
                    <list-item>
                      <p>Testimonial prompts for the teacher included the following:</p>
                    </list-item>
                  </list>
                  <list list-type="bullet">
                    <list-item>
                      <p>Tell us about yourself</p>
                    </list-item>
                    <list-item>
                      <p>How do you see LGBT+ students’ experiences in schools currently?</p>
                    </list-item>
                    <list-item>
                      <p>What is missing from our educational system in supporting LGBT+ students?</p>
                    </list-item>
                  </list>
                  <list list-type="bullet">
                    <list-item>
                      <p>What would you say to teachers to help them support their LGBT+ students?</p>
                    </list-item>
                  </list>
                </td>
              </tr>
              <tr valign="top">
                <td colspan="4">
                  <bold>Self-efficacy</bold>
                </td>
              </tr>
              <tr valign="top">
                <td>
                  <break/>
                </td>
                <td>Writing exercise, 5 minutes</td>
                <td>
                  <list list-type="bullet">
                    <list-item>
                      <p>Duration: 5 minutes 00 second</p>
                    </list-item>
                    <list-item>
                      <p>Timestamps: 43.45-48:45</p>
                    </list-item>
                  </list>
                </td>
                <td>
                  <list list-type="bullet">
                    <list-item>
                      <p>Writing prompt: “Imagine now that, as a teacher, you are interacting with the student you previously imagined. For example, the student asks for your help in a discussion after class. Knowing what you know now about the LGBT+ community and how we can help LGBT+ students, please imagine this interaction in which you offer suggestions and support. What would you say to this student? Please write down your answers.”</p>
                    </list-item>
                  </list>
                </td>
              </tr>
              <tr valign="top">
                <td colspan="2">Conclusion</td>
                <td>
                  <list list-type="bullet">
                    <list-item>
                      <p>Duration: 1 minutes 10 seconds</p>
                    </list-item>
                    <list-item>
                      <p>Timestamps: 48.45-49:55</p>
                    </list-item>
                  </list>
                </td>
                <td>
                  <list list-type="bullet">
                    <list-item>
                      <p>Acknowledging the advisory board representatives from the LGBT+ community for their input and feedback</p>
                    </list-item>
                  </list>
                </td>
              </tr>
            </tbody>
          </table>
          <table-wrap-foot>
            <fn id="table1fn1">
              <p><sup>a</sup>LGBT+: lesbian, gay, bisexual, and transgender people. The “+” symbol represents individuals whose identities do not fit typical binary notions of male and female (nonbinary).</p>
            </fn>
            <fn id="table1fn2">
              <p><sup>b</sup>All intervention elements are drawn from empirical evidence. We present timestamps and specific content which is included in the multimedia intervention resource.</p>
            </fn>
            <fn id="table1fn3">
              <p><sup>c</sup>UNICEF: United Nations Children's Fund.</p>
            </fn>
          </table-wrap-foot>
        </table-wrap>
      </sec>
    </sec>
    <sec sec-type="methods">
      <title>Methods</title>
      <sec>
        <title>Participants and Recruitment</title>
        <p>We plan to collect data exclusively from teachers or counselors working in Romanian public schools. All participants will be required to speak Romanian because of the language used in the intervention and measures. Participants who complete the intervention will be sent a participation certificate which could be used for continuing education credits and are given the option to enter a raffle where they can win a 500 RON (equivalent to US $110) gift certificate.</p>
        <p>Our recruitment strategy is to ask the local Center for Educational Resources and Assistance to distribute a message nationally, to all their members who are teachers or counselors employed in Romanian schools. If interested, they have the opportunity to sign up for 1 of the several sessions depending on their availability. The sessions are capped at 30, with an average of 25 participants signing up for each (range 24-27). We will advertise a total of 17 sessions across 3 months at different times during the day.</p>
      </sec>
      <sec>
        <title>Study Design</title>
        <p>This intervention’s experimental design is an RCT. We use a 2-group random assignment design, such that participants are randomly assigned to either the experimental condition in which they receive the intervention first and then complete the outcome measures, or to a control condition in which they complete the outcome measures first and then receive the intervention. From an ethical perspective, we chose this design so that all participants will receive the intervention and associated resources by the end of their participation. From a validity perspective, the outcome measures of those in the control condition are not affected by the intervention, thus serving as an appropriate control comparison to participants in the experimental condition.</p>
        <p>Participants will join sessions in groups of up to 30 participants, depending on their availability. Cluster randomization will be done at the session level, such that each session will be randomly assigned to either experimental or control conditions. Individual randomization within the session is not possible given that the intervention will be presented to all participants at the same time. Sessions will be scheduled across different times and days outside typical working hours, so we do not anticipate any systematic biases arising from participants’ session choices. To ensure experimental control, each session will be led by 2 researchers who deliver scripted instructions, answer questions, and ensure all participants complete the study at a similar pace and with minimal distraction.</p>
      </sec>
      <sec>
        <title>Intervention</title>
        <p>The intervention is designed for internet-based delivery and is multimedia, containing a recorded presentation, animations, testimonials of LGBT+ people, as well as 2 exercises. Whereas the intervention is rooted in empirical evidence, we also ensured cultural sensitivity by collaborating with local LGBT+ educational and advocacy nongovernmental organizations who offered feedback on intervention components. A detailed summary of the evidence-based components, as well as timestamps and duration for each subcomponent, are included in <xref ref-type="table" rid="table1">Table 1</xref>.</p>
      </sec>
      <sec>
        <title>Ethical Considerations</title>
        <p>Ethics approval was obtained from the West University of Timisoara, Romania, ethical panel (74505/10.11.2022), based on an application containing the procedure, measures, and materials used. Upon being presented with detailed information regarding the study, informed consent will be obtained from all participants before starting their participation. All data will be anonymously collected, with no personal or identifiable information being recorded. All fully anonymous data will be stored on password-protected computers and servers. Compensation consists of a participation certificate which could be used for continuing education credits and the option to enter a raffle where they can win a 500 RON (equivalent to US $110) gift certificate. There is no identification of individual participants in any images within the paper.</p>
      </sec>
      <sec>
        <title>Outcomes</title>
        <sec>
          <title>Overview</title>
          <p>With 1 exception (the factual knowledge variable), we will use validated scales to measure intervention outcomes. Where possible, we will use previously translated and validated scales in Romanian, selected from ResearchCentral repository, a free internet-based platform dedicated to the development of Romanian psychology by providing researchers with free, validated assessment tools. Where translations did not previously exist, 2 Romanian researchers, who are fluent in English, translated and then back-translated the scales to ensure accuracy. For all scales, before computing a final score, we will ensure sufficient reliability by computing Cronbach α, with a cutoff minimal score of 0.70.</p>
        </sec>
        <sec>
          <title>Attitudinal</title>
          <sec>
            <title>Overview</title>
            <p>Given the wide divergence in the definition of antigay bias or homophobia, several scales have been developed across time [<xref ref-type="bibr" rid="ref18">18</xref>] and will be used in this research.</p>
          </sec>
          <sec>
            <title>Attitudes Toward Lesbians and Gay Men Scale</title>
            <p>This 10-item scale measures beliefs and attitudes toward gay men and lesbians (“Sex between two men is just plain wrong” and “Female homosexuality is a perversion”) [<xref ref-type="bibr" rid="ref19">19</xref>]. Items are rated on a Likert scale from 1 (strongly disagree) to 5 (strongly agree). After reverse coding 4 items, scores will be averaged into a final score with higher values denoting more negative attitudes.</p>
          </sec>
          <sec>
            <title>Homophobia Scale</title>
            <p>This 25-item scale measures attitudes but also social avoidance and aggression toward gay people (“If I discovered a friend was gay I would end the friendship” and “I tease and make jokes about gay people”) [<xref ref-type="bibr" rid="ref20">20</xref>]. Items are rated from 1 (strongly disagree) to 5 (strongly agree) on a Likert scale. After reverse coding 9 items, scores will be averaged into a final score with higher values denoting more negative attitudes.</p>
          </sec>
          <sec>
            <title>Attitudes Toward Homosexuals Scale</title>
            <p>This 12-item scale measures attitudes toward gay people (“Homosexuality is disgusting in the eyes of God” and “If I can, I prefer to not be in the company of homosexuals”) [<xref ref-type="bibr" rid="ref21">21</xref>]. Items are rated from 1 (strongly disagree) to 5 (strongly agree) Likert scale. After reverse coding 5 items, scores will be averaged into a final score with higher values denoting more negative attitudes.</p>
          </sec>
        </sec>
        <sec>
          <title>Behavioral</title>
          <sec>
            <title>Behavioral Intentions</title>
            <p>This 16-item scale measures intentions for supportive professional behaviors that teachers would perform in the classroom (“I would talk with a student about questions regarding sexual orientation” and “I would have books about gay and lesbian issues in my classroom”) [<xref ref-type="bibr" rid="ref22">22</xref>,<xref ref-type="bibr" rid="ref23">23</xref>]. Items are rated on a Likert scale from 1 (strongly disagree) to 5 (strongly agree). Scores will be averaged into a final score with higher values denoting more willingness to engage in LGBT+ supportive behaviors in the classroom.</p>
          </sec>
          <sec>
            <title>Self-Efficacy</title>
            <p>This 10-item scale adapted the original scale to working with LGBT+ students in the school context (“If I try hard, I can solve difficult issues related to LGBT+ students” and “I can deal with unexpected situations that arise with LGBT+ students”) [<xref ref-type="bibr" rid="ref24">24</xref>]. Items were rated from 1 (Not at all true for me) to 4 (Perfectly true for me). Scores will be averaged into a final score with higher values denoting more self-efficacy in dealing with LGBT+-related behaviors in the classroom.</p>
          </sec>
        </sec>
        <sec>
          <title>Cognitive: Factual Knowledge About LGBT+ Issues</title>
          <p>We constructed 7 items based on training content to assess participants’ knowledge about LGBT+ issues (“Gender is a biological construct, unrelated to cultural associations” and “Heterosexual youth are 1.5 to 3 times more likely to attempt suicide compared to LGBT+ youth”). Items are rated as true or false, and a final score will be computed by adding up the number of correct responses, with higher scores denoting more knowledge about LGBT+ issues.</p>
        </sec>
        <sec>
          <title>Affective</title>
          <sec>
            <title>Feeling Thermometer (1 Item Each for Gay, Lesbian, and Bisexual)</title>
            <p>We will use a feeling thermometer [<xref ref-type="bibr" rid="ref25">25</xref>] to assess participants’ feelings toward gay, lesbian, and bisexual people by asking them to rate how they feel about each group using a slider thermometer scale from 0 (very negative feeling) to 100 (very positive feeling).</p>
          </sec>
          <sec>
            <title>Perspective Taking</title>
            <p>This 5-item measure assesses the extent to which participants take the perspective of LGBT+ people (“Can you imagine how an LGBT+ person feels?” and “Do you have an understanding of issues that are important for LGBT+ people”) on a scale from 1 (Never) to 5 (All the time) [<xref ref-type="bibr" rid="ref26">26</xref>]. Scores will be computed by averaging the 5 items, with higher final scores denoting more perspective-taking.</p>
          </sec>
          <sec>
            <title>Intergroup Disgust Sensitivity</title>
            <p>We adapted this 7-item scale to measure repulsion toward LGBT+ groups (“I feel disgusted when people with a different sexual orientation invade my personal space” and “After shaking hands with someone who has a different sexual orientation, even if their hands were clean, I would want to wash my hands.”) [<xref ref-type="bibr" rid="ref27">27</xref>]. Items are rated from 1 (strongly disagree) to 5 (strongly agree) on a Likert scale. After reverse scoring 1 item, scores will be averaged into a final score with higher values denoting more disgust toward LGBT+ outgroups.</p>
          </sec>
          <sec>
            <title>Intergroup Anxiety</title>
            <p>This 10-item scale measures anxiety-related emotions when interacting with people of another sexual orientation by asking them to rate their likelihood of feeling several emotions (embarrassed, unsure, irritated, suspicious, etc) on a scale from 1 (not at all) to 10 (extremely) [<xref ref-type="bibr" rid="ref28">28</xref>]. A total of 2 scores will be computed, 1 for positive emotions and another for negative emotions.</p>
          </sec>
          <sec>
            <title>Toronto Empathy Questionnaire</title>
            <p>This 16-item scale measures the general tendency to empathize with other people (“It upsets me to see someone being treated disrespectfully” and “I find that I am ‘in tune’ with other people’s moods”) [<xref ref-type="bibr" rid="ref29">29</xref>,<xref ref-type="bibr" rid="ref30">30</xref>]. Items are rated on a Likert scale from 1 (strongly disagree) to 5 (strongly agree). After reverse scoring 7 items, scores will be averaged into a final score with higher values denoting more empathy.</p>
          </sec>
        </sec>
        <sec>
          <title>Demographic and Control Variables</title>
          <sec>
            <title>Demographics</title>
            <p>It consists of age, gender, and sexual orientation.</p>
          </sec>
          <sec>
            <title>Contact With LGBT+ People</title>
            <p>We will ask participants how often they have contact (eg, talk) with a gay man, a lesbian woman, a bisexual woman or man, or a transgender person on a scale from 1 (almost daily) to 6 (never).</p>
          </sec>
          <sec>
            <title>Religiosity</title>
            <p>Religiosity (Duke University Religion Index) [<xref ref-type="bibr" rid="ref31">31</xref>] is a 5-item scale measure of religious involvement, measuring organizational religious activity, nonorganizational religious activity, and intrinsic religiosity (or subjective religiosity). Responses are averaged in 1 single score with higher scores denoting more religiosity.</p>
          </sec>
          <sec>
            <title>Ideology</title>
            <p>Ideology was measured by asking people to indicate their political orientation on a 100-point sliding scale from 0 (very conservative) to 50 (center) to 100 (very liberal or progressive).</p>
          </sec>
        </sec>
      </sec>
      <sec>
        <title>Statistical Analysis</title>
        <sec>
          <title>Power</title>
          <p>To determine our sample size, we conducted a power analysis using an average effect size of <italic>d</italic>=0.66 computed from Bartoş et al’s [<xref ref-type="bibr" rid="ref9">9</xref>] meta-analysis to ensure a statistical power of 0.80. The analysis indicated a sample of 122, but we aim to overrecruit, if possible, up to 200 participants given multiple outcomes. We do not have a stopping rule—we will recruit until all interested participants are given the chance to participate within 1 of the 17 sessions posted.</p>
        </sec>
        <sec>
          <title>Data Exclusion</title>
          <p>We will use data from all participants who completed their participation in the study, without any exclusions. Before computing final scores for each outcome variable, we will ensure sufficient reliability using Cronbach α, using a threshold of 0.70. To test our hypotheses, we will perform a series of 1-way between-subjects ANOVAs to compare attitudinal, cognitive, behavioral, and affective outcomes between the participants in the experimental and control conditions. For the effective measures we will perform the analyses (1) while controlling for contact with LBGT+ individuals and (2) separately by LGBT+ status (given the nature of the measures). We will report <italic>F</italic> test and <italic>P</italic> values, as well as all descriptive statistics (n, mean, and SD). We will also compute Cohen <italic>d</italic> for estimating the effect size, using means and SDs. Violin plots in R ggplot2 will also be included to visually represent means, CIs, as well as score distributions for each outcome.</p>
        </sec>
      </sec>
    </sec>
    <sec sec-type="results">
      <title>Results</title>
      <p>This paper details the protocol for the teacher intervention study. Data collection began in December 2022 and was completed by February 2023. Data analysis will be performed upon protocol acceptance. Follow-up measures will be completed in 2024. Results are expected to be submitted for publication following analysis in the spring of 2024.</p>
    </sec>
    <sec sec-type="discussion">
      <title>Discussion</title>
      <p>This paper describes the research protocol design and planned evaluation of an RCT aimed at improving attitudinal, cognitive, behavioral, and affective outcomes in Romanian teachers regarding LGBT+ inclusion. Strengths of the intervention include its evidence-based nature—all components included were derived from research showing positive outcomes on LGBT+ or other bias reduction. All elements were, however, adapted to be appropriate and sensitive to the Romanian cultural system and educational context. The intervention was also engaging and multimedia, thus increasing engagement. Importantly, it offers evidence-based tools to further address LGBT+ biases and bullying in schools, thus, potentially leading to stronger behavioral effects.</p>
      <p>Limitations include the fact that outcomes will be assessed immediately after the intervention, so we are unsure about the long-term effects, as well as whether potential positive effects further impact LGBT+ students’ lives. Further studies should investigate the effects on a larger sample of teachers, with a wider range of initial attitudes toward LGBT+ inclusion.</p>
      <p>If successful, however, the intervention has the potential of becoming a valuable, nationally available resource for teachers and high school counselors across Romania. The materials developed, especially those around education, perspective-taking, and self-efficacy can be further used to educate students about LGBT+ issues and increase their willingness and capacity to support LGBT+ peers. The findings resulting from this protocol are important, as they are the first to test the effectiveness of an evidence-based intervention in improving LGBT+ stigma in Romanian schools.</p>
    </sec>
  </body>
  <back>
    <app-group/>
    <glossary>
      <title>Abbreviations</title>
      <def-list>
        <def-item>
          <term id="abb1">LGBT+</term>
          <def>
            <p>lesbian, gay, bisexual, and transgender people. The “+” symbol represents individuals whose identities do not fit typical binary notions of male and female (nonbinary)</p>
          </def>
        </def-item>
        <def-item>
          <term id="abb2">RCT</term>
          <def>
            <p>randomized controlled trial</p>
          </def>
        </def-item>
        <def-item>
          <term id="abb3">UNESCO</term>
          <def>
            <p>The United Nations Educational, Scientific and Cultural Organization</p>
          </def>
        </def-item>
      </def-list>
    </glossary>
    <ack>
      <p>The research leading to these results has received funding from the Norway grants 2014-2021 (17⁄2020). The authors thank Sebastian Bartos, Lavinia Chirica, and Andre Rădulescu for their advice and assistance in developing the intervention.</p>
    </ack>
    <notes>
      <sec>
        <title>Data Availability</title>
        <p>The data sets generated and analyzed during this study will be published in anonymous form on Open Science Framework and the European Commission Funded Research (OpenAire) community platform. This will be done after the successful publication of the results.</p>
      </sec>
    </notes>
    <fn-group>
      <fn fn-type="conflict">
        <p>None declared.</p>
      </fn>
    </fn-group>
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